http://www.cnr.it/ontology/cnr/individuo/prodotto/ID277583
AN ALLERGEN-POLYMERIC NANOAGGREGATE AS A NEW TOOL FOR ALLERGY VACCINATION (Articolo in rivista)
- Type
- Label
- AN ALLERGEN-POLYMERIC NANOAGGREGATE AS A NEW TOOL FOR ALLERGY VACCINATION (Articolo in rivista) (literal)
- Anno
- 2014-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1016/j.ijpharm.2014.01.031 (literal)
- Alternative label
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Mariano Licciardi
Giovanna Montana
Maria Luisa Bondì
Angela Bonura
Cinzia Scialabba
Mario Raphael Melis
Calogero Fiorica
Gaetano Giammona
Paolo Colombo (literal)
- Pagina inizio
- Pagina fine
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
- Rivista
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- UNIPA
IBIM-CNR
ISMN-CNR (literal)
- Titolo
- AN ALLERGEN-POLYMERIC NANOAGGREGATE AS A NEW TOOL FOR ALLERGY VACCINATION (literal)
- Abstract
- Parietaria pollen is one of the major causes of allergic reaction in southern Europe,
affecting about 30% of all allergic patients in this area. Specifi immunotherapy is the only
treatment able to modify the natural outcome of the disease by restoring a normal immunity
against allergens. The preparation of allergen-solid lipid nanoparticles as delivery vehicles for
therapeutic proteins, P. judaica major allergen Par j 2, was investigated. The Par j 2 allergen was
expressed in a large amount in Escherichia coli and purifid to homogeneity. Its immunological
properties were studied by western blotting and enzyme-linked immunosorbent assay inhibition.
Solid lipid nanoparticles were obtained by water-in-oil-in-water multiple emulsion method and
characterized in terms of mean size and surface charge. These systems (approximately 250 nm
diameter and negative surface charge) incorporated recombinant Par j 2 with 40% or greater
effiiency. Moreover, the endotoxin level and anaphylactic activity of the empty solid lipid
nanoparticles and recombinant Par j 2-loaded solid lipid nanoparticles were evaluated by looking
at the overexpression of CD203c marker on human basophils. These results demonstrate that
recombinant Par j 2-nanoparticles could be proposed as safe compositions for the development
of new therapeutic dosage forms to cure allergic reactions. (literal)
- Prodotto di
- Autore CNR
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