AN ALLERGEN-POLYMERIC NANOAGGREGATE AS A NEW TOOL FOR ALLERGY VACCINATION (Articolo in rivista)

Type
Label
  • AN ALLERGEN-POLYMERIC NANOAGGREGATE AS A NEW TOOL FOR ALLERGY VACCINATION (Articolo in rivista) (literal)
Anno
  • 2014-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1016/j.ijpharm.2014.01.031 (literal)
Alternative label
  • Mariano Licciardi Giovanna Montana Maria Luisa Bondì Angela Bonura Cinzia Scialabba Mario Raphael Melis Calogero Fiorica Gaetano Giammona Paolo Colombo (2014)
    AN ALLERGEN-POLYMERIC NANOAGGREGATE AS A NEW TOOL FOR ALLERGY VACCINATION
    in International journal of pharmaceutics (Print)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Mariano Licciardi Giovanna Montana Maria Luisa Bondì Angela Bonura Cinzia Scialabba Mario Raphael Melis Calogero Fiorica Gaetano Giammona Paolo Colombo (literal)
Pagina inizio
  • 275 (literal)
Pagina fine
  • 283 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 465 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
  • 1-2 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • UNIPA IBIM-CNR ISMN-CNR (literal)
Titolo
  • AN ALLERGEN-POLYMERIC NANOAGGREGATE AS A NEW TOOL FOR ALLERGY VACCINATION (literal)
Abstract
  • Parietaria pollen is one of the major causes of allergic reaction in southern Europe, affecting about 30% of all allergic patients in this area. Specifi immunotherapy is the only treatment able to modify the natural outcome of the disease by restoring a normal immunity against allergens. The preparation of allergen-solid lipid nanoparticles as delivery vehicles for therapeutic proteins, P. judaica major allergen Par j 2, was investigated. The Par j 2 allergen was expressed in a large amount in Escherichia coli and purifid to homogeneity. Its immunological properties were studied by western blotting and enzyme-linked immunosorbent assay inhibition. Solid lipid nanoparticles were obtained by water-in-oil-in-water multiple emulsion method and characterized in terms of mean size and surface charge. These systems (approximately 250 nm diameter and negative surface charge) incorporated recombinant Par j 2 with 40% or greater effiiency. Moreover, the endotoxin level and anaphylactic activity of the empty solid lipid nanoparticles and recombinant Par j 2-loaded solid lipid nanoparticles were evaluated by looking at the overexpression of CD203c marker on human basophils. These results demonstrate that recombinant Par j 2-nanoparticles could be proposed as safe compositions for the development of new therapeutic dosage forms to cure allergic reactions. (literal)
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