Multifunctional aptamer-miRNA conjugates for targeted cancer therapy. (Articolo in rivista)

Type
Label
  • Multifunctional aptamer-miRNA conjugates for targeted cancer therapy. (Articolo in rivista) (literal)
Anno
  • 2014-01-01T00:00:00+01:00 (literal)
Alternative label
  • Esposito CL, Cerchia L, Catuogno S, de Vita G, Dassie JP, Santamaria G, Swiderski P, Condorelli G, Giangrande PH, and de Franciscis V (2014)
    Multifunctional aptamer-miRNA conjugates for targeted cancer therapy.
    in Molecular therapy (Print)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Esposito CL, Cerchia L, Catuogno S, de Vita G, Dassie JP, Santamaria G, Swiderski P, Condorelli G, Giangrande PH, and de Franciscis V (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • 1Istituto di Endocrinologia ed Oncologia Sperimentale, CNR, Naples, Italy 2Department of Internal Medicine, University of Iowa, Iowa City, IA 52242, USA 3Laboratorio di Medicina Molecolare e Genomica, University of Salerno, Baronissi, Salerno, Italy 4Synthetic and Biopolymer Chemistry Core, Beckman research Institute of City of Hope, Duarte, CA, USA 5Dipartimento di Medicina Molecolare e Biotecnologie Mediche, University of Naples \"Federico II\", Naples, Italy 6Molecular & Cellular Biology Program, University of Iowa, Iowa City, IA 52242, USA (literal)
Titolo
  • Multifunctional aptamer-miRNA conjugates for targeted cancer therapy. (literal)
Abstract
  • While miRNAs clearly regulate multiple pathways integral to disease development and progression, the lack of safe and reliable means for specific delivery of miRNAs to target tissues represents a major obstacle to their broad therapeutic application. Our objective was to explore the use of nucleic acid aptamers as carriers for cell-targeted delivery of a miRNA with tumor suppressor function, let-7g. Using an aptamer that binds to and antagonizes the oncogenic receptor tyrosine kinase Axl (GL21.T), here we describe the development of aptamer-miRNA conjugates as multifunctional molecules that inhibit growth of Axl-expressing tumors. We conjugated the let-7g miRNA to GL21.T and demonstrate selective delivery to target cells, processing by the RNAi machinery, and silencing of let-7g target genes. Importantly, the multifunctional conjugate reduced tumor growth in a xenograft model of lung adenocarcinoma. Therefore, our data establish aptamer-miRNA conjugates as a novel tool for targeted delivery of miRNAs with therapeutic potential.Molecular Therapy (2014); doi:10.1038/mt.2014.5. (literal)
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