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Chlorhexidine binding to mineralized versus demineralized dentin powder. (Articolo in rivista)

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Chlorhexidine binding to mineralized versus demineralized dentin powder. (Articolo in rivista) (literal)

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Kim J, Uchiyama T, Carrilho M, Agee KA, Mazzoni A, Breschi L, Carvalho RM, Tjäderhane L, Looney S, Wimmer C, Tezvergil-Mutluay A, Tay FR, Pashley DH. (2010)
Chlorhexidine binding to mineralized versus demineralized dentin powder.
in Dental materials
(literal)

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Kim J, Uchiyama T, Carrilho M, Agee KA, Mazzoni A, Breschi L, Carvalho RM, Tjäderhane L, Looney S, Wimmer C, Tezvergil-Mutluay A, Tay FR, Pashley DH. (literal)

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Department of Conservative Dentistry, School of Dentistry, Kyung Hee National University, Seoul, Republic of Korea; Department of Regenerative Dentistry, Nihon University School of Dentistry at Matsuso, Chiba, Japan; GEO/UNIBAN, Health Institute, Bandeirante University of São Paulo, Brazil Department of Dental Materials and Oral Biochemistry, University of São Paulo, School of Dentistry, São Paulo/SP, Brazil; School of Dentistry, Medical College of Georgia, Augusta, GA, USA; Department of SAU & FAL, University of Bologna, Bologna, Italy; Department of Biomedicine, University of Trieste, Italy - IGM-CNR, Unit of Bologna, c/o IOR, Bologna, Italy; Department of Operative Dentistry, College of Dentistry, University of Florida, Gainesville, FL, USA; Institute of Dentistry, University of Oulu and Oulu University Hospital, Oulu, Finland; Department of Biostatistics, Medical College of Georgia, Augusta, GA, USA; Department of Prosthodontics, School of Dentistry, University of Turku, Turku, Finland; Department of Endodontics, School of Dentistry, Medical College of Georgia, Augusta, GA, USA (literal)

Titolo

Chlorhexidine binding to mineralized versus demineralized dentin powder. (literal)

Abstract

OBJECTIVES: The purposes of this work were to quantitate the affinity and binding capacity of chlorhexidine (CHX) digluconate to mineralized versus demineralized dentin powder and to determine how much debinding would result from rinsing with water, ethanol, hydroxyethylmethacrylate (HEMA) or 0.5M NaCl in water. METHODS: Dentin powder was made from coronal dentin of extracted human third molars. Standard amounts of dentin powder were tumbled with increasing concentrations of CHX (0-30 mM) for 30 min at 37 degrees C. After centrifuging the tubes, the supernatant was removed and the decrease in CHX concentration quantitated by UV-spectroscopy. CHX-treated dentin powder was resuspended in one of the four debinding solutions for 3 min. The amount of debound CHX in the solvents was also quantitated by UV-spectroscopy. RESULTS: As the CHX concentration in the medium increased, the CHX binding to mineralized dentin powder also increased up to 6.8 micromol/g of dry dentin powder. Demineralized dentin powder took up significantly (p<0.01) more CHX, reaching 30.1 micromol CHX/g of dry dentin powder. Debinding of CHX was in the order: HEMA

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