Identification of a functional nuclear export sequence in diacylglycerol kinase-zeta (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Identification of a functional nuclear export sequence in diacylglycerol kinase-zeta (Articolo in rivista) (literal)

Anno

• 2010-01-01T00:00:00+01:00 (literal)

Alternative label

• Evangelisti C, Gaboardi GC, Billi AM, Ognibene A, Goto K, Tazzari PL, McCubrey JA, Martelli AM. (2010)
  Identification of a functional nuclear export sequence in diacylglycerol kinase-zeta
  in Cell cycle (Georget. Tex.)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Evangelisti C, Gaboardi GC, Billi AM, Ognibene A, Goto K, Tazzari PL, McCubrey JA, Martelli AM. (literal)

Pagina inizio

• 384 (literal)

Pagina fine

• 388 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 9 (literal)

Rivista

• Cell cycle (Rivista)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Dipartimento di Scienze Anatomiche Umane e Fisiopatologia dell’Apparato Locomotore; Cell Signaling Laboratory; Università di Bologna; Bologna, Italy; Laboratorio di Biologia Cellulare e Microscopia Elettronica; IOR; Bologna, Italy; Department of Anatomy and Cell Biology; Yamagata University School of Medicine; Yamagata, Japan; Servizio Immunoematologia e Trasfusionale; Policlinico S.Orsola-Malpighi; Bologna, Italy; Department of Microbiology and Immunology; Brody School of Medicine; East Carolina University; Greenville, NC USA; IGM-CNR; Unità di Bologna c/o IOR; Bologna, Italy (literal)

Titolo

• Identification of a functional nuclear export sequence in diacylglycerol kinase-zeta (literal)

Abstract

• Diacylglycerol kinases (DGKs) are key regulators of diacylglycerol-dependent signaling pathways. Among the 10 DGK isoforms, DGK-zeta is the only nuclear form that contains a nuclear localization signal. Here, by site-directed mutagenesis, we showed that DGK-zeta also displays a functional independent nuclear export signal (NES) sequence between the amino acid residues 362-370. Indeed, the NES mutant forms of DGK-zeta accumulated in the nucleus to a much greater extent than wildtype DGK-zeta. Moreover, treatment with leptomycin B, an inhibitor of leucine-rich type NES, resulted in accumulation of both endogenous and ectopically expressed DGK-zeta in the nucleus, demonstrating that nuclear export of DGK-zeta is chromosome regional maintenance protein 1 (CRM1)-dependent. Previously, we reported that nuclear DGK-zeta is a negative regulator of cell cycle progression in C2C12 mouse myoblasts. In this paper, we documented that enhancement of DGK-zeta nuclear localization by NES sequence mutation, increases G(0)/G(1) block in C2C12 cells. Overall, our data demonstrate that DGK-zeta export from nucleus to cytoplasm is regulated by a leucine-rich NES through the exportin CRM1 and suggest that the nuclear localization of DGK-zeta could finely tune its function as a regulator of G(1)/S cell cycle transition. (literal)

Prodotto di

• Institute of molecular genetics (IGM) (Istituto)
• Patogenesi delle malattie degenerative muscolo-scheletriche (ME.P05.014.001) (Modulo)

Autore CNR

• Alberto Maria Martelli (Persona)

Insieme di parole chiave

• Keywords of "Identification of a functional nuclear export sequence in diacylglycerol kinase-zeta" (Insieme di parole chiave)

Incoming links:

Prodotto

• Institute of molecular genetics (IGM) (Istituto)
• Patogenesi delle malattie degenerative muscolo-scheletriche (ME.P05.014.001) (Modulo)

Autore CNR di

• Alberto Maria Martelli (Persona)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Cell cycle (Rivista)

Insieme di parole chiave di

• Keywords of "Identification of a functional nuclear export sequence in diacylglycerol kinase-zeta" (Insieme di parole chiave)