Leukocyte Elastase Inhibitor: a new regulator of PARP-1. (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Leukocyte Elastase Inhibitor: a new regulator of PARP-1. (Articolo in rivista) (literal)

Anno

• 2009-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1111/j.1749-6632.2009.04701 (literal)

Alternative label

• Lepretre C, Sidoli G, Scovassi AI, Torriglia A. (2009)
  Leukocyte Elastase Inhibitor: a new regulator of PARP-1.
  in Annals of the New York Academy of Sciences
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Lepretre C, Sidoli G, Scovassi AI, Torriglia A. (literal)

Pagina inizio

• 25 (literal)

Pagina fine

• 31 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 1171 (literal)

Rivista

• Annals of the New York Academy of Sciences (Rivista)

Note

• Scopus (literal)
• ISI Web of Science (WOS) (literal)
• PubMe (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• IGM, INSERM, Parigi, Francia (literal)

Titolo

• Leukocyte Elastase Inhibitor: a new regulator of PARP-1. (literal)

Abstract

• Poly(ADP-ribose) polymerase-1 (PARP-1) is an important regulator of apoptosis. Its over-activation at the onset of apoptosis can inhibit the action of apoptotic endonucleases like Caspase-activated DNase and DNAS1L3. Therefore, controlled PARP-1 proteolysis during caspase-dependent apoptosis is considered essential to promote DNA degradation. Yet, little is known about the interplay of PARP-1 and endonucleases that operate during caspase-independent cell death. Here we show that in the long-term cultured HeLa cells which undergo caspase-independent death, PARP-1 co-immunoprecipitates with Leukocyte Elastase Inhibitor-derived DNase II (L-DNaseII), an acid DNase implicated in this death pathway and activated by serine proteases. Our results indicate that, despite having putative poly(ADP-ribose)-acceptor sites, LEI/L-DNase II is neither significantly poly(ADP-ribosyl)ated nor inhibited by PARP-1 during caspase-independent apoptosis. Unexpectedly, caspase-independent apoptosis induced by Hexa-methylene amiloride, LEI/L-DNase II can activate PARP-1 and promote its auto-poly(ADP-ribosyl)ation, thus inhibiting PARP-1 activity. Moreover, overexpression of LEI blocks the pro-survival effect of PARP-1 in this model of cell death. Our results provide the original evidence for a new mechanism of PARP-1 activity regulation in the caspase-independent death pathway involving LEI/L-DNase II. (literal)

Prodotto di

• Institute of molecular genetics (IGM) (Istituto)
• Cromosomi artificiali per terapia genica. Studi sulla replicazione del DNA in cellule umane ; controllo del ciclo cellulare e della proliferazione. (ME.P05.006.001) (Modulo)

Autore CNR

• ANNA SCOVASSI (Unità di personale interno)

Insieme di parole chiave

• Keywords of "Leukocyte Elastase Inhibitor: a new regulator of PARP-1." (Insieme di parole chiave)

Incoming links:

Prodotto

• Institute of molecular genetics (IGM) (Istituto)
• Cromosomi artificiali per terapia genica. Studi sulla replicazione del DNA in cellule umane ; controllo del ciclo cellulare e della proliferazione. (ME.P05.006.001) (Modulo)

Autore CNR di

• ANNA SCOVASSI (Unità di personale interno)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Annals of the New York Academy of Sciences (Rivista)

Insieme di parole chiave di

• Keywords of "Leukocyte Elastase Inhibitor: a new regulator of PARP-1." (Insieme di parole chiave)