DNA-ligand binding mode discrimination by characterizing fluorescence resonance energy transfer through lifetime measurements with picosecond resolution (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• DNA-ligand binding mode discrimination by characterizing fluorescence resonance energy transfer through lifetime measurements with picosecond resolution (Articolo in rivista) (literal)

Anno

• 2008-01-01T00:00:00+01:00 (literal)

Alternative label

• Nardo, L; Bondani, M; Andreoni, A (2008)
  DNA-ligand binding mode discrimination by characterizing fluorescence resonance energy transfer through lifetime measurements with picosecond resolution
  in Photochemistry and photobiology
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Nardo, L; Bondani, M; Andreoni, A (literal)

Pagina inizio

• 101 (literal)

Pagina fine

• 110 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 84 (literal)

Rivista

• Photochemistry and photobiology (Rivista)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• \"[Nardo, Luca; Andreoni, Alessandra] Univ Insubria, Dipartimento Matemat & Fis, Como, Italy; [Bondani, Maria] INFM, CNR, Natl Lab Ultrafast & Ultraintense Opt Sci, Como, Italy (literal)

Titolo

• DNA-ligand binding mode discrimination by characterizing fluorescence resonance energy transfer through lifetime measurements with picosecond resolution (literal)

Abstract

• We describe a method for distinguishing between minor groove binders and base intercalators that is based on measurements of the fluorescence lifetime of a donor (D) in the presence of an acceptor (A). The D-A pair is separated by a short double helix DNA with which the ligands interact. By plotting the D fluorescence lifetime as a function of the ligand-to-base pair concentration ratio we find a clear signature that distinguishes between the two binding mechanisms: minor groove binding induces an asymptotic decrease of the D fluorescence lifetime, while intercalation gives a monotonically increasing lifetime and the appearance of an additional short lifetime. We assayed Quinacrine, Hoechst and 4'-6'diamidine-2-phenyl indole, which in control experiments performed on oligodeoxyribonucleotides (oligos) lacking the A are demonstrated not to interfere with the D fluorescence. The changes in fluorescence lifetimes measured in the case of dual-labeled oligos are thus caused by structural changes in the DNA that modify the D-A distance. The appearance of the short-lived transient in the fluorescence decay of Ds attached to dual-labeled oligos upon binding of an intercalator can be interpreted as denaturation. (literal)

Prodotto di

• Fotonica ultrabreve dall'infrarosso al EUV: applicazioni a materiali e dispositivi per ICT, magnetismo, biomedicina e nanoscienze (MD.P03.008.001) (Modulo)

Autore CNR

• MARIA BONDANI (Persona)

Insieme di parole chiave

• Keywords of "DNA-ligand binding mode discrimination by characterizing fluorescence resonance energy transfer through lifetime measurements with picosecond resolution" (Insieme di parole chiave)

Incoming links:

Autore CNR di

• MARIA BONDANI (Persona)

Prodotto

• Fotonica ultrabreve dall'infrarosso al EUV: applicazioni a materiali e dispositivi per ICT, magnetismo, biomedicina e nanoscienze (MD.P03.008.001) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Photochemistry and photobiology (Rivista)

Insieme di parole chiave di

• Keywords of "DNA-ligand binding mode discrimination by characterizing fluorescence resonance energy transfer through lifetime measurements with picosecond resolution" (Insieme di parole chiave)