Apoptotic DNA fragmentation can be revealed in situ: An ultrastructural approach (Articolo in rivista)

Type
Label
  • Apoptotic DNA fragmentation can be revealed in situ: An ultrastructural approach (Articolo in rivista) (literal)
Anno
  • 2009-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1002/jemt.20735 (literal)
Alternative label
  • Burattini S, Ferri P, Battistelli M, D'emilio A, Biagiotti L, Sestili P, Rocchi MB, Falcieri E. (2009)
    Apoptotic DNA fragmentation can be revealed in situ: An ultrastructural approach
    in Microscopy research and technique (Print)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Burattini S, Ferri P, Battistelli M, D'emilio A, Biagiotti L, Sestili P, Rocchi MB, Falcieri E. (literal)
Pagina inizio
  • 913 (literal)
Pagina fine
  • 923 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 72 (literal)
Rivista
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Di.SUAN, Università degli Studi di Urbino \"Carlo Bo\", I-61029 Urbino, Italy; Laboratorio di Biologia Cellulare e Microscopia Elettronica, Istituti Ortopedici Rizzoli, I-40136 Bologna, Italy; Dipartimento di Scienze Biomolecolari, Universita` degli Studi di Urbino \"Carlo Bo\", I-61029 Urbino, Italy; IGM, CNR, Istituti Ortopedici Rizzoli, I-40136 Bologna, Italy (literal)
Titolo
  • Apoptotic DNA fragmentation can be revealed in situ: An ultrastructural approach (literal)
Abstract
  • A common pattern of apoptotic death is DNA cleavage, initially producing large fragments (50 kbp), followed by the production of nucleosomic/oligonucleosomic fragments. Nevertheless, apoptosis without DNA fragmentation, at least of the nucleosomic type, has been reported. To investigate the spatial relationship between DNA cleavage and chromatin condensation, we applied the TUNEL technique to the ultrastructural analysis of apoptotic cells. A modified method, utilizing a gold-conjugated antidigoxigenin antibody, was carried out on U937 versus Molt-4 cells, both exposed to UVB radiation or staurosporine treatment. Gold particle density in the different domains of apoptotic cells was evaluated by a four-way ANOVA test. Gold labelling was more strongly localised in condensed chromatin than in the diffuse chromatin. U937 cells, which evidenced in vitro oligonucleosomic fragmentation after both UVB and staurosporine treatments, revealed a significantly higher gold particle density, when compared with Molt-4, which did not show, on the other hand, oligonucleosomic cleavage even in the presence of
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