http://www.cnr.it/ontology/cnr/individuo/prodotto/ID27638

Immunohistochemical and biochemical assay of versican in human sound predentine/dentine matrix. (Articolo in rivista)

Type

Articolo in rivista (Classe)
Prodotto della ricerca (Classe)

Label

Immunohistochemical and biochemical assay of versican in human sound predentine/dentine matrix. (Articolo in rivista) (literal)

Anno

2009-01-01T00:00:00+01:00 (literal)

Alternative label

Ruggeri A, Orsini G, Mazzoni A, Nato F, Papa V, Piccirilli M, Putignano A, Mazzotti G, De Stefano Dorigo E, Breschi L. (2009)
Immunohistochemical and biochemical assay of versican in human sound predentine/dentine matrix.
in European journal of histochemistry (Testo stamp.)
(literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

Ruggeri A, Orsini G, Mazzoni A, Nato F, Papa V, Piccirilli M, Putignano A, Mazzotti G, De Stefano Dorigo E, Breschi L. (literal)

Pagina inizio

125 (literal)

Pagina fine

134 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

53 (literal)

Rivista

European journal of histochemistry (Rivista)

Note

ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

Department of SAU&FAL, University of Bologna, Italy; Department of Clinical Sciences and Stomatology, Polytechnic University of Marche, Ancona, Italy; Department of SUAN, University of Urbino, Urbino Italy; Department of Stomatology and Oral Science, University of Chieti-Pescara, Italy; Unit of Dental Sciences and Biomaterials, Department of Biomedicine, University of Trieste; IGM-CNR, Unit of Bologna c/o IOR, Bologna, Italy (literal)

Titolo

Immunohistochemical and biochemical assay of versican in human sound predentine/dentine matrix. (literal)

Abstract

Aim of this study was to investigate the distribution of versican proteoglycan within the human dentine organic matrix by means of a correlative immunohistochemical analysis with field emission in-lens scanning electron microscope (FEI-SEM), transmission electron microscope (TEM), fluorescence microscope (FM) and biochemical assay. Specimens containing dentine and predentine were obtained from non carious human teeth and divided in three groups: 1) FEI-SEM group: sections were exposed to a pre-embedding immunohistochemical procedure; 2) TEM group: specimens were fixed, demineralised, embedded and submitted to a post-embedding immunohistochemical procedure; 3) FM group: sections mineralised and submitted to a pre-embedding immunohistochemical procedure with fluorescence labelling. Specimens were exposed to two different antibodies to assay distribution of versican fragments and whole versican molecule.Western Blotting analysis of dentine and pulp extracts was also performed. The correlative FEI-SEM,TEM and FM analysis revealed positive immunoreaction for versican fragments both in predentine and dentine, while few gold particles identifying the whole versican molecule were found in predentine only under TEM. No labelling of versican whole molecule was detected by FEI-SEM and FM analysis. The immunoblotting analysis confirmed the morphological findings. This study suggests that in fully developed human teeth versican fragments are significant constituents of the human dentine and predentine organic matrix, while versican whole molecule can be visualised in scarce amount within predentine only. The role of versican fragments within human dentine organic matrix should be further elucidated. (literal)

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