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CNBP regulates wing development in Drosophila melanogaster by promoting IRES-dependent translation of dMYC (Articolo in rivista)

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CNBP regulates wing development in Drosophila melanogaster by promoting IRES-dependent translation of dMYC (Articolo in rivista) (literal)

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Antonucci Laura; D'Amico Davide; Di Magno Laura; Coni Sonia; Di Marcotullio Lucia; Cardinali Beatrice; Gulino Alberto; Ciapponi Laura; Canettieri Gianluca (2014)
CNBP regulates wing development in Drosophila melanogaster by promoting IRES-dependent translation of dMYC
in Cell cycle (Georget. Tex.)
(literal)

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Antonucci Laura; D'Amico Davide; Di Magno Laura; Coni Sonia; Di Marcotullio Lucia; Cardinali Beatrice; Gulino Alberto; Ciapponi Laura; Canettieri Gianluca (literal)

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Laura Antonucci: Department of Molecular Medicine; Sapienza University; Rome, Italy; Istituto Pasteur - Fondazione Cenci Bolognetti; Rome, Italy. Davide D'Amico: Department of Molecular Medicine; Sapienza University; Rome, Italy. Laura Di Magno: Department of Molecular Medicine; Sapienza University; Rome, Italy; Istituto Pasteur - Fondazione Cenci Bolognetti; Rome, Italy. Sonia Coni: Istituto Pasteur - Fondazione Cenci Bolognetti; Rome, Italy; CNRS UMR 7277; INSERM 1091; Institut de Biologie de Valrose (iBV); Université de Nice-Sophia Antipolis; Nice, France. Lucia Di Marcotullio: Department of Molecular Medicine; Sapienza University; Rome, Italy. Beatrice Cardinali: Cellular Biology and Neurobiology Institute; IBCN; National Research Council; Monterotondo, Rome, Italy. Alberto Gulino: Department of Molecular Medicine; Sapienza University; Rome, Italy; Istituto Pasteur - Fondazione Cenci Bolognetti; Rome, Italy; Neuromed Institute; Pozzilli, Italy; Center for Life NanoScience at LaSapienza; Istituto Italiano di Tecnologia; Rome, Italy. Laura Ciapponi: Department of Biology and Biotechnologies; Sapienza University; Rome, Italy. Gianluca Canettieri: Department of Molecular Medicine; Sapienza University; Rome, Italy. (literal)

Titolo

CNBP regulates wing development in Drosophila melanogaster by promoting IRES-dependent translation of dMYC (literal)

Abstract

CCHC-type zinc finger nucleic acid binding protein (CNBP) is a small conserved protein, which plays a key role in development and disease. Studies in animal models have shown that the absence of CNBP results in severe developmental defects that have been mostly attributed to its ability to regulate c-Myc mRNA expression. Functionally, CNBP binds single-stranded nucleic acids and acts as a molecular chaperone, thus regulating both transcription and translation. In this work we report that in Drosophila melanogaster, CNBP is an essential gene, whose absence causes early embryonic lethality. In contrast to what observed in other species, ablation of CNBP does not affect dMyc mRNA expression, whereas the protein levels are markedly reduced. We demonstrate for the first time that dCNBP regulates dMyc translation through an IRES-dependent mechanism, and that knockdown of dCNBP in the wing territory causes a general reduction of wing size, in keeping with the reported role of dMyc in this region. Consistently, reintroduction of Myc in CNBP-deficient wing imaginal discs rescues the wing size, further supporting a key role of the CNBP-Myc axis in this context. Collectively, these data show a previously uncharacterized mechanism, whereby, by regulating dMyc IRES-dependent translation, CNBP controls Drosophila wing development. These results may have relevant implications in other species and in pathophysiological condition (literal)

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