Gene amplification, radiation sensitivity and DNA double strand breaks (Articolo in rivista)

  • Gene amplification, radiation sensitivity and DNA double strand breaks (Articolo in rivista) (literal)
  • 2010-01-01T00:00:00+01:00 (literal)
  • 10.1016/j.mrrev.2010.01.008 (literal)
Alternative label
  • Mondello C; Smirnova A; Giulotto E. (2010)
    Gene amplification, radiation sensitivity and DNA double strand breaks
    in Mutation research. Reviews in mutation research (Print)
  • Mondello C; Smirnova A; Giulotto E. (literal)
Pagina inizio
  • 29 (literal)
Pagina fine
  • 37 (literal)
  • 704 (literal)
  • Questo lavoro è una review dei dati della letteratura che riguardano i meccanismi ed il ruolo dell'amplificazione genica nelle cellule di mammifero. Particolare interesse è rivolto alle rotture a doppia elica del DNA come eventi di innesco dell'amplificazione ed al ruolo dei meccanismi di riparazione del DNA nella formazione del DNA amplificato. (literal)
  • 9 (literal)
  • 1-3 (literal)
  • PubMe (literal)
  • Scopu (literal)
  • ISI Web of Science (WOS) (literal)
  • Mondello C: Istituto di Genetica Molecolare, CNR, Pavia Smirnova A, Giulotto E: Dipartimento di Genetica e Microbiologia, Università di Pavia, Pavia. (literal)
  • Gene amplification, radiation sensitivity and DNA double strand breaks (literal)
  • DNA double strand breaks (DSBs) are one of the main type of damage induced by ionizing radiations. Free DNA ends that are not correctly repaired can be engaged in pathways triggering gene amplification. Following gene amplification the copy number of a portion of the genome is increased, leading to an enhanced expression of the genes located in the amplified region. Gene amplification plays an important role in cancer, being one of the mechanisms of oncogene activation; in addition, it can confer resistance to chemotherapeutic agents, through the increase in the copy number of genes coding for drug targets. The presence of gene amplification can have a prognostic and a diagnostic value and can help in orienting therapy in specific tumour types. The amplified DNA is primarily produced through recombination-based pathways and can be located either within chromosomes or on extra-chromosomal acentric elements. Studies on the organization of the amplified DNA in tumour cells and in cultured drug resistant cells have suggested that a single DSB can trigger a cascade of events leading to a large number of copies of a region of the genome. In addition, it has been shown that amplified DNA is unstable, further increasing the long-term effect of the initial event. Gene amplification is a peculiar feature of transformed cells and the ability to amplify is strongly influenced by the cellular genetic background. Genes involved in DNA damage response and in DNA damage repair can play a role in controlling the amplification process, in particular, it has been shown that defects in DSB repair functions can increase the frequency of gene amplification. In this review, we will discuss the biological significance of gene amplification, together with the role of DNA DSBs and DSB repair genes in the generation of amplified DNA. Copyright © 2010. Published by Elsevier B.V. (literal)
Prodotto di
Autore CNR
Insieme di parole chiave

Incoming links:

Autore CNR di
Insieme di parole chiave di