p63 regulates glutaminase 2 expression (Articolo in rivista)

Type
Label
  • p63 regulates glutaminase 2 expression (Articolo in rivista) (literal)
Anno
  • 2013-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.4161/cc.24478 (literal)
Alternative label
  • Giacobbe Arianna1; Bongiorno-Borbone Lucilla1; Bernassola Francesca1; Terrinoni Alessandro2; Markert Elke Katrin3; Levine Arnold J.3; Feng Zhaohui4; Agostini Massimiliano5; Zolla Lello6; Finazzi Agrò Alessandro1; Notterman Daniel A.7, Melino Gerry8,9; Peschiaroli Angelo10 (2013)
    p63 regulates glutaminase 2 expression
    in Cell cycle (Georget. Tex.)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Giacobbe Arianna1; Bongiorno-Borbone Lucilla1; Bernassola Francesca1; Terrinoni Alessandro2; Markert Elke Katrin3; Levine Arnold J.3; Feng Zhaohui4; Agostini Massimiliano5; Zolla Lello6; Finazzi Agrò Alessandro1; Notterman Daniel A.7, Melino Gerry8,9; Peschiaroli Angelo10 (literal)
Pagina inizio
  • 1395 (literal)
Pagina fine
  • 1405 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#altreInformazioni
  • Epub 2013 Apr 10 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 12 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
  • 9 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • 1 Department of Experimental Medicine and Surgery; University of Rome \"Tor Vergata\"; Rome, Italy. 2 IDI-IRCCS Biochemistry Laboratory c/o Department of Experimental Medicine and Surgery; University of Rome \"Tor Vergata\"; Rome, Italy. 3 The Simons Center for Systems Biology; Institute for Advanced Study; Princeton, NJ USA. 4 Cancer Institute of New Jersey; University of Medicine and Dentistry of New Jersey; New Brunswick, NJ USA. 5 Medical Research Council; Toxicology Unit; Leicester University; Leicester, UK. 6 Department of Enviromental Sciences; University of Tuscia; Largo dell'Università; Viterbo, Italy. 7 College of Medicine; Pennsylvania State University; Hershey, PA USA. 8 IDI-IRCCS Biochemistry Laboratory c/o Department of Experimental Medicine and Surgery; University of Rome \"Tor Vergata\"; Rome, Italy. 9 Medical Research Council; Toxicology Unit; Leicester University; Leicester, UK. 10 Institute of Cellular Biology and Neurobiology; CNR; Rome, Italy. (literal)
Titolo
  • p63 regulates glutaminase 2 expression (literal)
Abstract
  • The transcription factor p63 is critical for many biological processes, including development and maintenance of epidermal tissues and tumorigenesis. Here, we report that the TAp63 isoforms regulate cell metabolism through the induction of the mitochondrial glutaminase 2 (GLS2) gene both in primary cells and tumor cell lines. By ChIP analysis and luciferase assay, we confirmed that TAp63 binds directly to the p53/p63 consensus DNA binding sequence within the GLS2 promoter region. Given the critical role of p63 in epidermal differentiation, we have investigated the regulation of GLS2 expression during this process. GLS2 and TAp63 expression increases during the in vitro differentiation of primary human keratinocytes, and depletion of GLS2 inhibits skin differentiation both at molecular and cellular levels. We found that GLS2 and TAp63 expression are concomitantly induced in cancer cells exposed to oxidative stresses. siRNA-mediated depletion of GLS2 sensitizes cells to ROS-induced apoptosis, suggesting that the TAp63/GLS2 axis can be functionally important as a cellular antioxidant pathway in the absence of p53. Accordingly, we found that GLS2 is upregulated in colon adenocarcinoma. Altogether, our findings demonstrate that GLS2 is a bona fide TAp63 target gene, and that the TAp63-dependent regulation of GLS2 is important for both physiological and pathological processes. (literal)
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