http://www.cnr.it/ontology/cnr/individuo/prodotto/ID276061
Reverse engineering a mouse embryonic stem cell-specific transcriptional network reveals a new modulator of neuronal differentiation. (Articolo in rivista)
- Type
- Label
- Reverse engineering a mouse embryonic stem cell-specific transcriptional network reveals a new modulator of neuronal differentiation. (Articolo in rivista) (literal)
- Anno
- 2013-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1093/nar/gks1136 (literal)
- Alternative label
De Cegli R, Iacobacci S, Flore G, Gambardella G, Mao L, Cutillo L, Lauria M, Klose J, Illingworth E, Banfi S, di Bernardo D. (2013)
Reverse engineering a mouse embryonic stem cell-specific transcriptional network reveals a new modulator of neuronal differentiation.
in Nucleic acids research
(literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- De Cegli R, Iacobacci S, Flore G, Gambardella G, Mao L, Cutillo L, Lauria M, Klose J, Illingworth E, Banfi S, di Bernardo D. (literal)
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- Rivista
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- Note
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- Telethon Institute of Genetics and Medicine, Via P. Castellino 111,
CNR Institute of Genetics and Biophysics \"Adriano Buzzati Traverso\", Napoli 80131, Italy,
Institut für Humangenetik Charité, Campus Virchow-Klinikum, Universitätsmedizin Berlin, Augustenburger Platz 1, Berlin D-13353, Germany,
Department of Statistics and Mathematics for the Economic Research, University of Naples \"Parthenope\", Via Acton 38, Naples 80133,
Microsoft Research-University of Trento Centre for Computational and Systems Biology, Piazza Giannantonio Manci 17, Trento 38050,
Universita' degli Studi di Salerno, Via Ponte don Melillo, Fisciano, Salerno 84084,
Medical Genetics, Department of General Pathology, Second University of Naples, Via L. De Crecchio 7, Naples 80138 and Faculty of Engineering, University of Naples \"Federico II\", Piazzale Tecchio 80, Naples 80125, Italy (literal)
- Titolo
- Reverse engineering a mouse embryonic stem cell-specific transcriptional network reveals a new modulator of neuronal differentiation. (literal)
- Abstract
- Gene expression profiles can be used to infer previously unknown transcriptional regulatory interaction among thousands of genes, via systems biology 'reverse engineering' approaches. We 'reverse engineered' an embryonic stem (ES)-specific transcriptional network from 171 gene expression profiles, measured in ES cells, to identify master regulators of gene expression ('hubs'). We discovered that E130012A19Rik (E13), highly expressed in mouse ES cells as compared with differentiated cells, was a central 'hub' of the network. We demonstrated that E13 is a protein-coding gene implicated in regulating the commitment towards the different neuronal subtypes and glia cells. The overexpression and knock-down of E13 in ES cell lines, undergoing differentiation into neurons and glia cells, caused a strong up-regulation of the glutamatergic neurons marker Vglut2 and a strong down-regulation of the GABAergic neurons marker GAD65 and of the radial glia marker Blbp. We confirmed E13 expression in the cerebral cortex of adult mice and during development. By immuno-based affinity purification, we characterized protein partners of E13, involved in the Polycomb complex. Our results suggest a role of E13 in regulating the division between glutamatergic projection neurons and GABAergic interneurons and glia cells possibly by epigenetic-mediated transcriptional regulation. (literal)
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