Fermented Wheat Powder Induces the Antioxidant and Detoxifying System in Primary Rat Hepatocytes (Articolo in rivista)

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  • Fermented Wheat Powder Induces the Antioxidant and Detoxifying System in Primary Rat Hepatocytes (Articolo in rivista) (literal)
Anno
  • 2013-01-01T00:00:00+01:00 (literal)
Alternative label
  • Margherita La Marca, Pascale Beffy, Annalisa Pugliese, Vincenzo Longo (2013)
    Fermented Wheat Powder Induces the Antioxidant and Detoxifying System in Primary Rat Hepatocytes
    in PloS one
    (literal)
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  • Margherita La Marca, Pascale Beffy, Annalisa Pugliese, Vincenzo Longo (literal)
Pagina inizio
  • 1 (literal)
Pagina fine
  • 9 (literal)
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  • 8 (literal)
Rivista
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  • 12 (literal)
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  • Istituto di Biologia e Biotecnologia Agraria, CNR, Pisa, Italy, Istituto di Fisiologia Clinica, CNR, Pisa, Italy (literal)
Titolo
  • Fermented Wheat Powder Induces the Antioxidant and Detoxifying System in Primary Rat Hepatocytes (literal)
Abstract
  • Abstract Many plants exhibit antioxidant properties which may be useful in the prevention of oxidative stress reactions, such as those mediated by the formation of free radical species in different pathological situations. In recent years a number of studies have shown that whole grain products in particular have strong antioxidant activity. Primary cultures of rat hepatocytes were used to investigate whether and how a fermented powder of wheat (Lisosan G) is able to modulate antioxidant and detoxifying enzymes, and whether or not it can activate Nrf2 transcription factor or inhibit NF-kB activation. All of the antioxidant and detoxifying enzymes studied were significantly up-regulated by 0.7 mg/ml Lisosan G treatment. In particular, NAD(P)H:quinone oxidoreductase and heme oxygenase-1 were induced, although to different degrees, at the transcriptional, protein and/or activity levels by the treatment. As for the Nrf2 transcription factor, a partial translocation of its protein from the cytosol to the nucleus after 1 h of Lisosan G treatment was revealed by immunoblotting. Lisosan G was also observed to decrease H2O2-induced toxicity Taken together, these results show that this powder of wheat is an effective inducer of ARE/Nrf2-regulated antioxidant and detoxifying genes and has the potential to inhibit the translocation of NF-kB into the nucleus. Citation: La Marca M, Beffy P, Pugliese A, Longo V (2013) Fermented Wheat Powder Induces the Antioxidant and Detoxifying System in Primary Rat Hepatocytes. PLoS ONE 8(12): e83538. doi:10.1371/journal.pone.0083538 Editor: Ben C. B. Ko, The Hong Kong Polytechnic University, Hong Kong Received June 21, 2013; Accepted November 5, 2013; Published December 31, 2013 Copyright: ? 2013 La Marca et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Funding: No current external funding sources for this study. Competing Interests: Agrisan Company supplied Lisosan G used in this study. There are no further patents, products in development or marketed products to declare. This does not alter the authors' adherence to all the PLOS ONE policies on sharing data and materials. (literal)
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