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2-DE and LC-MS/MS for a comparative proteomic analysis of BALf from subjects with different subsets of inflammatory myopathies. (Articolo in rivista)

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2-DE and LC-MS/MS for a comparative proteomic analysis of BALf from subjects with different subsets of inflammatory myopathies. (Articolo in rivista) (literal)

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Passadore I, Iadarola P, Di Poto C, Giuliano S, Montecucco C, Cavagna L, Bonino C, Meloni F, Fietta AM, Lisa A, Salvini R, Bardoni AM. (2009)
2-DE and LC-MS/MS for a comparative proteomic analysis of BALf from subjects with different subsets of inflammatory myopathies.
in Journal of proteome research (Print)
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Passadore I, Iadarola P, Di Poto C, Giuliano S, Montecucco C, Cavagna L, Bonino C, Meloni F, Fietta AM, Lisa A, Salvini R, Bardoni AM. (literal)

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Department of Biochemistry \"A. Castellani\", University of Pavia, Via Taramelli 3/B, 27100 Pavia, Italy, Division of Rheumatology, University of Pavia, Via Taramelli 5, 27100 Pavia, Italy, Department of Haematological, Pneumological and Cardiovascular Sciences, University of Pavia, Via Taramelli 5, 27100 Pavia, Italy, IRCCS San Matteo Foundation, Piazzale Golgi 2, 27100 Pavia, Italy, Division of Rheumatology, Immunology and Allergy, Georgetown University Proteomics Laboratory, Washington, D.C. 20057, and Institute of Molecular Genetics, CNR, 27100 Pavia, Italy (literal)

Titolo

2-DE and LC-MS/MS for a comparative proteomic analysis of BALf from subjects with different subsets of inflammatory myopathies. (literal)

Abstract

The protein profiles of bronchoalveolar lavage fluid (BALf) of patients belonging to three selected subsets of Polymyositis/Dermatomyositis (PM/DM) have been compared by using a combination of 2-DE and MALDI-TOF/MS or LC-MS/MS. Our study examined the hypothesis that there were distinct differences in protein expression profiles that were related to the phenotype. From among the 323 51 protein spots that may represent the most highly expressed proteins in BALf of these patients, 24 unique spots were isolated and proteins identified. In particular, 9 spots were present in BALf of PM/DM patients only; 12 spots were exclusive of Overlap patients and 3 spots of AS patients. From among the proteins identified, a few were classified as cytoskeletal proteins, others were involved in oxidative stress and a number of proteins were associated with general metabolic activity or immunological response and inflammation. This is the first study in which evidence is provided that a number of different proteins are expressed in different subsets of PM/DM and supports our contention that the proteomic approach would be beneficial in discovering molecules which could represent possible prognostic factors of these rare pathologies. (literal)

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