Phytosiderophore Release of Barley Plants under Iron Deficiency in Hydroponic and Soil Conditions: Comparison of Analytical Methods (Contributo in atti di convegno)

Type
Label
  • Phytosiderophore Release of Barley Plants under Iron Deficiency in Hydroponic and Soil Conditions: Comparison of Analytical Methods (Contributo in atti di convegno) (literal)
Anno
  • 2013-01-01T00:00:00+01:00 (literal)
Alternative label
  • R. Fijan(1), F. Valentinuzzi(1), R. Terzano(2), L. Medici(3), D. Del Buono(4), N. Tomasi(5), R. Pinton(5), S. Cesco(1) and T. Mimmo(1) (2013)
    Phytosiderophore Release of Barley Plants under Iron Deficiency in Hydroponic and Soil Conditions: Comparison of Analytical Methods
    in ICOBTE 2013: 12th International Conference on the Biogeochemistry of Trace Elements, Athens, Georgia, USA, 16.06.2013 - 20.06.2013
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • R. Fijan(1), F. Valentinuzzi(1), R. Terzano(2), L. Medici(3), D. Del Buono(4), N. Tomasi(5), R. Pinton(5), S. Cesco(1) and T. Mimmo(1) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • (1) Faculty of Science and Technology, Free University of Bolzano, Bolzano, Italy Rebeka.Fijan@unibz.it; (2) Department of Soil, Plant and Food Sciences, University of Bari \"Aldo Moro\", Bari, Italy; (3) Institute of Methodologies for Environmental Analysis, C.N.R., Tito Scalo, Potenza, Italy; (4) Department of Agricultural and Environmental Sciences, University of Perugia, Perugia, Italy; (5) Department of Agricultural and Environmental Sciences, University of Udine, Udine, Italy (literal)
Titolo
  • Phytosiderophore Release of Barley Plants under Iron Deficiency in Hydroponic and Soil Conditions: Comparison of Analytical Methods (literal)
Abstract
  • Iron (Fe) is an essential plant nutrient usually present in the soil mainly in insoluble Fe(III) forms. Although it is one of the major soil constituents (0.5 to 5%), plant availability is severely limited by the low solubility of Fe hydroxides at pH levels favorable for plant growth. Therefore, plants need special mechanisms for acquiring Fe from sparingly soluble Fe forms to fit the requirements for growth, especially in neutral and alkaline soils, where the availability of Fe is particularly low. In response to Fe-deficiency, graminaceous plants (Strategy II plants) are able to release considerable amounts of non-proteinaceous amino acids, so called phytosiderophores of the mugineic-acid family, which are highly effective chelators for Fe(III) and have an important function in the mobilization and uptake of Fe. In this research barley seedlings (Hordeum vulgare L. cv Europa) were grown hydroponically in Fe-free nutrient solution using a plant-based soil biotest (RHIZOtest) until the first visible symptoms of chlorosis, followed by a six day plant-soil test period. 15-day old Fe-deficient plants were put in close contact with various calcareous soils separating the plant roots from soil by a 30-m polyamide mesh to facilitate the collection of root exudates, rhizosphere soil and plant tissues. For the duration of the plant-soil contact period, other Fe-deficient plants were kept growing hydroponically and root exudates were collected every three days. Phytosiderophores extracted from the soils and collected from hydroponically grown plants will be determined quantitatively, using an indirect colorimetric method (Cu-CAS assay), and qualitatively by an innovative reversed phase HPLC method with pre-column derivatization and fluorescence detection. The effects of phytosiderophores on soil mineralogy will be assessed by XRD (X-Ray Diffraction) and SEM-EDX (Scanning Electron Microscopy coupled to Energy Dispersive X-ray spectroscopy). The efficiency of the mobilization and acquisition mechanisms will be evaluated measuring the accumulation of nutrients in plant tissues via ICP measurements. The combination of innovative techniques will enable a better understanding and definition of the efficiency of root exudates in modifying the soil characteristics in order to increase the availability of micronutrients in the rhizosphere. Moreover, the sampling and quali-quantification of root exudates in both soil and hydroponic solution will allow the evaluation of the soil influence on the release of these compounds. Finally, the results will enable the assessment of capability of grassy plants to acquire the poorly soluble Fe. (literal)
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