AKAP149 binds to HIV-1 reverse transcriptase and is involved in the reverse transcription. (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• AKAP149 binds to HIV-1 reverse transcriptase and is involved in the reverse transcription. (Articolo in rivista) (literal)

Anno

• 2008-01-01T00:00:00+01:00 (literal)

Alternative label

• Lemay J, Maidou-Peindara P, Cancio R, Ennifar E, Coadou G, Maga G, Rain JC, Benarous R, Liu LX. (2008)
  AKAP149 binds to HIV-1 reverse transcriptase and is involved in the reverse transcription.
  in Journal of Molecular Biology
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Lemay J, Maidou-Peindara P, Cancio R, Ennifar E, Coadou G, Maga G, Rain JC, Benarous R, Liu LX. (literal)

Pagina inizio

• 783 (literal)

Pagina fine

• 796 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 383 (literal)

Rivista

• Journal of Molecular Biology (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#descrizioneSinteticaDelProdotto

• full paper (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Institut Cochin, Université Paris Descartes, CNRS (UMR 8104), Paris, France; Inserm, U567, Paris, France; Institute of Molecular Genetics–CNR, National Research Council, via Abbiategrasso 207, I-27100 Pavia, Italy; Architecture et Réactivité de l'ARN, UPR 9002 CNRS, 15 rue René Descartes, 67084 Strasbourg, France; Laboratory of Nuclear Magnetic Resonance, European Institute for Peptide Research (IFRMP 23), UMR 6014 CNRS, University of Rouen, 76821 Mont-Saint-Aignan, France Hybrigenics S.A., F-75014 Paris, France (literal)

Titolo

• AKAP149 binds to HIV-1 reverse transcriptase and is involved in the reverse transcription. (literal)

Abstract

• Like all retroviruses, human immunodeficiency virus type 1 (HIV-1) undergoes reverse transcription during its replication cycle. The cellular cofactors potentially involved in this process still remain to be identified. We show here that A-kinase anchoring protein 149 (AKAP149) interacts with HIV-1 reverse transcriptase (RT) in both the yeast two-hybrid system and human cells. The AKAP149 binding site has been mapped to the RNase H domain of HIV-1 RT. AKAP149 silencing by RNA interference in HIV-1-infected cells inhibited viral replication at the reverse transcription step. We selected single-point mutants of RT defective for AKAP149 binding and demonstrated that mutant G462R, despite retaining significant intrinsic RT activity in vitro, failed to carry out HIV-1 reverse transcription correctly in infected cells. This suggests that the interaction between RT and AKAP149 in infected cells may play an important role in HIV-1 reverse transcription. (literal)

Prodotto di

• Sviluppo e meccanismo d'azione di analoghi nucleotidici e nucleosidici come composti antiproliferativi e antivirali : nuovi composti e nuovi bersagli per la terapia (ME.P04.004.001) (Modulo)
• Institute of molecular genetics (IGM) (Istituto)

Autore CNR

• GIOVANNI MAGA (Unità di personale interno)
• REYNEL CANCIO FERNANDEZ (Unità di personale esterno)

Insieme di parole chiave

• Keywords of "AKAP149 binds to HIV-1 reverse transcriptase and is involved in the reverse transcription." (Insieme di parole chiave)

Incoming links:

Prodotto

• Institute of molecular genetics (IGM) (Istituto)
• Sviluppo e meccanismo d'azione di analoghi nucleotidici e nucleosidici come composti antiproliferativi e antivirali : nuovi composti e nuovi bersagli per la terapia (ME.P04.004.001) (Modulo)

Autore CNR di

• GIOVANNI MAGA (Unità di personale interno)
• REYNEL CANCIO FERNANDEZ (Unità di personale esterno)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Journal of Molecular Biology (Rivista)

Insieme di parole chiave di

• Keywords of "AKAP149 binds to HIV-1 reverse transcriptase and is involved in the reverse transcription." (Insieme di parole chiave)