http://www.cnr.it/ontology/cnr/individuo/prodotto/ID275048
Validation study on urinary biomarkers of exposure for aflatoxin B1, ochratoxin A, fumonisin B1, deoxynivalenol and zearalenone in piglet. (Articolo in rivista)
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- Validation study on urinary biomarkers of exposure for aflatoxin B1, ochratoxin A, fumonisin B1, deoxynivalenol and zearalenone in piglet. (Articolo in rivista) (literal)
- Anno
- 2013-01-01T00:00:00+01:00 (literal)
- Alternative label
Gambacorta L.1, M. Solfrizzo1, A. Visconti1, S. Powers2, A.M. Cossalter3,4, P. Pinton3,4 and I. P. Oswald3,4 (2013)
Validation study on urinary biomarkers of exposure for aflatoxin B1, ochratoxin A, fumonisin B1, deoxynivalenol and zearalenone in piglet.
in World mycotoxin journal (Online)
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- Gambacorta L.1, M. Solfrizzo1, A. Visconti1, S. Powers2, A.M. Cossalter3,4, P. Pinton3,4 and I. P. Oswald3,4 (literal)
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- 1Institute of Sciences of Food Production (ISPA), National Research Council of Italy (CNR), 70126 Bari, Italy; 2Vicam, A Waters Business, 34 Maple Street, Milford, MA 01757, USA; 3INRA, UMR1331, Toxalim, Research Centre in Food Toxicology, F-31027 Toulouse, France; 4Université de Toulouse, INP, UMR1331, Toxalim, F-31000 Toulouse, France; (literal)
- Titolo
- Validation study on urinary biomarkers of exposure for aflatoxin B1, ochratoxin A, fumonisin B1, deoxynivalenol and zearalenone in piglet. (literal)
- Abstract
- The multi-biomarker approach was used to validate urinary biomarkers in piglets administered with boluses contaminated with different doses of a mixture of deoxynivalenol (DON), aflatoxin B1 (AFB1), fumonisin B1 (FB1), zearalenone (ZEA) and ochratoxin A (OTA). Boluses contaminated with target levels of mycotoxins were prepared by slurrying with water and freeze-drying feed material fortified with extracts of culture materials of selected toxigenic fungi. Piglets were individually placed in metabolic cages to collect urine before gavage and in 24 h urine post dose. Urine samples were hydrolysed with ?-glucuronidase enzyme and analysed by a multi-biomarker LC-MS/MS method developed and validated to identify and measure biomarkers of FB1, OTA, DON, ZEA and AFB1. Urinary levels of FB1, OTA, DON + de-epoxy-deoxynivalenol (DOM-1), ZEA + alpha-zearalenol (?-ZOL) and aflatoxin M1 (AFM1), were selected as biomarkers of FB1, OTA, DON, ZEA and AFB1, respectively. Mean percentages of dietary mycotoxins excreted as biomarkers in the 24 h post-dose urines were 36.8% for ZEA, 28.5% for DON, 2.6% FB1, 2.6% for OTA and 2.5% for AFB1. A good correlation was observed between the amount of mycotoxins ingested and the amount of the relevant biomarkers excreted in 24 h post-dose urines. Linear dose-response correlation coefficients ranged between 0.68 to 0.78 for the tested couples mycotoxin/biomarker. The good sensitivity of the LC-MS/MS method and the good dose-response correlations observed in this study permitted to validate the selected mycotoxin biomarkers in piglet at dietary levels close to the maximum permitted levels reported in the directive 2003/100/EC for AFB1 and the guidance values reported in the recommendation 2006/576/EC for DON, ZEA, OTA and FB1. (literal)
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