Contribution of telomerase RNA retrotranscription to DNA double-strand break repair during mammalian genome evolution. (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Contribution of telomerase RNA retrotranscription to DNA double-strand break repair during mammalian genome evolution. (Articolo in rivista) (literal)

Anno

• 2007-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1186/gb-2007-8-12-r260 (literal)

Alternative label

• Nergadze SG, Santagostino MA, Salzano A, Mondello C, Giulotto E. (2007)
  Contribution of telomerase RNA retrotranscription to DNA double-strand break repair during mammalian genome evolution.
  in Genome biology (Online)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Nergadze SG, Santagostino MA, Salzano A, Mondello C, Giulotto E. (literal)

Pagina inizio

• R260.1 (literal)

Pagina fine

• R260.13 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 8 (literal)

Rivista

• Genome biology (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

• 13 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 12 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#descrizioneSinteticaDelProdotto

• Il lavoro mette in evidenza il ruolo della telomerasi nella riparazione delle rotture a doppia elica. (literal)

Note

• Scopu (literal)
• ISI Web of Science (WOS) (literal)
• Pubme (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• MOndello C:, Istituto di Genetica Molecolare del CNR di Pavia; Nergadze SG, Santagostino MA, Salzano A, Giulotto E: Dipartimento di Genetica e Microbiologia, Universita` degli Studi di Pavia, Pavia (literal)

Titolo

• Contribution of telomerase RNA retrotranscription to DNA double-strand break repair during mammalian genome evolution. (literal)

Abstract

• Background: In vertebrates, tandem arrays of TTAGGG hexamers are present at both telomeres and intrachromosomal sites (interstitial telomeric sequences (ITSs)). We previously showed that, in primates, ITSs were inserted during the repair of DNA double-strand breaks and proposed that they could arise from either the capture of telomeric fragments or the action of telomerase. Results: An extensive comparative analysis of two primate (Homo sapiens and Pan troglodytes) and two rodent (Mus musculus and Rattus norvegicus) genomes allowed us to describe organization and insertion mechanisms of all the informative ITSs present in the four species. Two novel observations support the hypothesis of telomerase involvement in ITS insertion: in a highly significant fraction of informative loci, the ITSs were introduced at break sites where a few nucleotides homologous to the telomeric hexamer were exposed; in the rodent genomes, complex ITS loci are present in which a retrotranscribed fragment of the telomerase RNA, far away from the canonical template, was inserted together with the telomeric repeats. Moreover, mutational analysis of the TTAGGG arrays in the different species suggests that they were inserted as exact telomeric hexamers, further supporting the participation of telomerase in ITS formation. Conclusion: These results strongly suggest that telomerase was utilized, in some instances, for the repair of DNA double-strand breaks occurring in the genomes of rodents and primates during evolution. The presence, in the rodent genomes, of sequencesretrotranscribed from the telomerase RNA strengthens the hypothesis of the origin of telomerase from an ancient retrotransposon. (literal)

Prodotto di

• Malattie genetiche dovute a difetti nella riparazione del DNA che predispongono ai tumori. Analisi genetica funzionale dei fattori importanti per l'integrità del genoma. (ME.P05.005.001) (Modulo)
• Institute of molecular genetics (IGM) (Istituto)

Autore CNR

• CHIARA MONDELLO (Unità di personale interno)

Insieme di parole chiave

• Parole chiave di "Contribution of telomerase RNA retrotranscription to DNA double-strand break repair during mammalian genome evolution." (Insieme di parole chiave)

Incoming links:

Prodotto

• Institute of molecular genetics (IGM) (Istituto)
• Malattie genetiche dovute a difetti nella riparazione del DNA che predispongono ai tumori. Analisi genetica funzionale dei fattori importanti per l'integrità del genoma. (ME.P05.005.001) (Modulo)

Autore CNR di

• CHIARA MONDELLO (Unità di personale interno)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Genome biology (Rivista)

Insieme di parole chiave di

• Parole chiave di "Contribution of telomerase RNA retrotranscription to DNA double-strand break repair during mammalian genome evolution." (Insieme di parole chiave)