Timing and expression level of protein kinase C epsilon regulate the megakaryocytic differentiation of human CD34 cells. (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Timing and expression level of protein kinase C epsilon regulate the megakaryocytic differentiation of human CD34 cells. (Articolo in rivista) (literal)

Anno

• 2007-01-01T00:00:00+01:00 (literal)

Alternative label

• Gobbi G, Mirandola P, Sponzilli I, Micheloni C, Malinverno C, Cocco L, Vitale M. (2007)
  Timing and expression level of protein kinase C epsilon regulate the megakaryocytic differentiation of human CD34 cells.
  in Stem cells (Dayt. Ohio)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Gobbi G, Mirandola P, Sponzilli I, Micheloni C, Malinverno C, Cocco L, Vitale M. (literal)

Pagina inizio

• 2322 (literal)

Pagina fine

• 2329 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 25 (literal)

Rivista

• Stem cells (Rivista)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Department of Anatomy, Pharmacology & Forensic Medicine, Human Anatomy Section, University of Parma, Ospedale Maggiore, via Gramsci, Parma, Italy; Cellular Signalling Laboratory, Department of Anatomical Sciences, University of Bologna, Bologna, Italy; Istituto di Genetica Molecolare-Consiglio Nazionale delle Ricerche, Unit of Bologna, c/o Istituti Ortopedici Rizzoli, Bologna, Italy. (literal)

Titolo

• Timing and expression level of protein kinase C epsilon regulate the megakaryocytic differentiation of human CD34 cells. (literal)

Abstract

• Protein kinase C (PKC)-mediated intracellular signaling participates in several key steps of hematopoietic cell differentiation. The epsilon isoform of PKC has been associated with erythroid differentiation as well as with the early phases of megakaryocytic (MK) lineage commitment. Here, we worked on the hypothesis that PKCepsilon expression levels might be modulated during MK differentiation, with a specific role in the early as well as in the late phases of thrombopoiesis. We demonstrate that--at variance with the erythroid lineage development--PKCepsilon is completely downmodulated in TPO-induced CD34 cells from day 6 onward. The forced expression of PKCepsilon in the late phases of MK differentiation delays the phenotypic differentiation of progenitors likely via Bcl-xL upregulation. Moreover, tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), known as a negative regulator of early erythroid expansion, is not apoptogenic for thrombopoietin-induced CD34 cells, but rather accelerates their maturation. However, PKCepsilon levels negatively interfere also with the effects of TRAIL in MK differentiation. PKCepsilon can therefore be considered a signaling intermediate whose expression levels are finely tuned, with a virtually opposite kinetic, in erythroid versus megakaryocytic lineages, to adequately respond to the signaling requirements of the specific hematopoietic lineage. (literal)

Prodotto di

• EFFETTI DELLE RADIAZIONI DI RISONANZA DI CICLOTRONE DI IONI SULLA PROLIFERAZIONE DI CELLULE STAMINALI EMATOPOIETICHE (ME.P06.012.005) (Modulo)
• Bersagli molecolari per il controllo della progressione tumorale (ME.P03.012.001) (Modulo)
• Institute of molecular genetics (IGM) (Istituto)

Autore CNR

• MARCO VITALE (Persona)

Incoming links:

Prodotto

• Institute of molecular genetics (IGM) (Istituto)
• EFFETTI DELLE RADIAZIONI DI RISONANZA DI CICLOTRONE DI IONI SULLA PROLIFERAZIONE DI CELLULE STAMINALI EMATOPOIETICHE (ME.P06.012.005) (Modulo)
• Bersagli molecolari per il controllo della progressione tumorale (ME.P03.012.001) (Modulo)

Autore CNR di

• MARCO VITALE (Persona)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Stem cells (Rivista)