Electromagnetic enhancement of a culture of human SAOS-2 osteoblasts seeded onto titanium fiber-mesh scaffolds. (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Electromagnetic enhancement of a culture of human SAOS-2 osteoblasts seeded onto titanium fiber-mesh scaffolds. (Articolo in rivista) (literal)

Anno

• 2008-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1002/jbm.a.31827 (literal)

Alternative label

• Fassina L, Saino E, Visai L, Silvani G, Cusella De Angelis MG, Mazzini G, Benazzo F, Magenes G. (2008)
  Electromagnetic enhancement of a culture of human SAOS-2 osteoblasts seeded onto titanium fiber-mesh scaffolds.
  in Journal of biomedical materials research. Part A
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Fassina L, Saino E, Visai L, Silvani G, Cusella De Angelis MG, Mazzini G, Benazzo F, Magenes G. (literal)

Pagina inizio

• 750 (literal)

Pagina fine

• 759 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 87 (literal)

Rivista

• Journal of biomedical materials research. Part A (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 3 (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Dipartimento di Informatica e Sistemistica, University of Pavia, Pavia, Italy; Dipartimento di Biochimica, University of Pavia, Pavia, Italy; Dipartimento di Medicina Sperimentale, University of Pavia, Pavia, Italy; IGM-CNR Istochimica e Citometria; Dip.Biol. Animale Università, Pavia, Italy; Dipartimento SMEC, IRCCS San Matteo, University of Pavia, Pavia, Italy; Centro di Ingegneria Tissutale (C.I.T.), University of Pavia, Pavia, Italy (literal)

Titolo

• Electromagnetic enhancement of a culture of human SAOS-2 osteoblasts seeded onto titanium fiber-mesh scaffolds. (literal)

Abstract

• The surface properties of a biomaterial are fundamental in order to determine the response of the host tissue. In the present study we have followed a particular biomimetic strategy where electromagnetically stimulated SAOS-2 human osteoblasts proliferated and built a calcified extracellular matrix on a titanium fiber-mesh surface. In comparison with control conditions, the electromagnetic stimulation (magnetic field intensity, 2 mT; frequency, 75 Hz) caused higher cell proliferation and increased surface coating with type-I collagen, decorin, and osteopontin (9.8-fold, 11.3-fold, and 9.5-fold, respectively). RT-PCR analysis revealed the electromagnetically upregulated transcription specific for the foregoing matrix proteins and for the growth factor TGF-b. The immunofluorescence of type-I collagen, decorin, and osteopontin showed their colocalization in the cell-rich areas. The use of an electromagnetic bioreactor aimed at obtaining the surface modification of the biocompatible metallic scaffold in terms of cell colonization and coating with calcified extracellular matrix. The superficially modified biomaterial could be used, in clinical applications, as an implant for bone repair. (literal)

Prodotto di

• Institute of molecular genetics (IGM) (Istituto)
• Sviluppi metodologici in citometria e applicazioni alla sperimentazione biomedica; in vitro imaging (ME.P06.010.001) (Modulo)

Autore CNR

• GIULIANO MAZZINI (Persona)

Insieme di parole chiave

• Keywords of "Electromagnetic enhancement of a culture of human SAOS-2 osteoblasts seeded onto titanium fiber-mesh scaffolds." (Insieme di parole chiave)

Incoming links:

Prodotto

• Institute of molecular genetics (IGM) (Istituto)
• Sviluppi metodologici in citometria e applicazioni alla sperimentazione biomedica; in vitro imaging (ME.P06.010.001) (Modulo)

Autore CNR di

• GIULIANO MAZZINI (Persona)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Journal of biomedical materials research. Part A (Rivista)

Insieme di parole chiave di

• Keywords of "Electromagnetic enhancement of a culture of human SAOS-2 osteoblasts seeded onto titanium fiber-mesh scaffolds." (Insieme di parole chiave)