SAFB re-distribution marks steps of the apoptotic process. (Articolo in rivista)

Type
Label
  • SAFB re-distribution marks steps of the apoptotic process. (Articolo in rivista) (literal)
Anno
  • 2007-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1016/j.yexcr.2007.06.023 (literal)
Alternative label
  • Lee YB; Colley S; Norman M; Biamonti G; Uney JB. (2007)
    SAFB re-distribution marks steps of the apoptotic process.
    in Experimental cell research; Elsevier, Amsterdam (Paesi Bassi)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Lee YB; Colley S; Norman M; Biamonti G; Uney JB. (literal)
Pagina inizio
  • 3914 (literal)
Pagina fine
  • 3923 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url
  • http://www.sciencedirect.com/science/article/pii/S0014482707002996 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 313 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali
  • 10 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
  • 18 (literal)
Note
  • PubMe (literal)
  • Scopu (literal)
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • LY-B,CS NM, UJB Henry Wellcome Laboratories for Integrative Neuroscience and Endocrinology (LINE), Bristol University, UK; CS: Laboratory for Cancer Medicine, Western Australia Institute for Medical Research, Perth, Australia; BG: Istituto di Genetica Molecolare CNR, Pavia, Italy. (literal)
Titolo
  • SAFB re-distribution marks steps of the apoptotic process. (literal)
Abstract
  • We have found novel functions of scaffold attachment factor-B1 (SAFB) during apoptosis. The experiments showed that SAFB moved into the nucleolus 15 min after the induction of apoptosis and before the release of cytochrome c into the cytoplasm. Two hours later SAFB formed a peri-nucleolar ring-like structure and this occurred after cytochrome c release and before PARP cleavage. Digestion with RNase suggested that the peri-nucleolar ring structure was dependent on RNA integrity and a RNA moiety formed part of this structure. Studies using SAFB deletion mutants showed that the formation of the peri-nucleolar structure was not mediated by the DNA binding (SAP) or the RNA binding (RRM) domain of SAFB but was instead dependent on the S/K and R/E coiled-coil regions: a result suggesting that the structure is formed via protein interactions. In addition, SAFB cleavage was shown to be mediated by caspase-3 and occurred after the formation of the peri-nucleolar ring and after cleavage of PARP (characteristic of proteins having a direct role in apoptosis). A determinant for this cleavage is located in the DNA binding domain and we hypothesize that SAFB may direct the reorganization and segregation of nuclear RNA and DNA prior to endonuclease-mediated DNA cleavage. (literal)
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