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Modifications of the acidic soluble salivary proteome in human children from birth to the age of 48 months investigated by a top-down HPLC-ESI-MS platform (Articolo in rivista)

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Modifications of the acidic soluble salivary proteome in human children from birth to the age of 48 months investigated by a top-down HPLC-ESI-MS platform (Articolo in rivista) (literal)

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Alternative label

Manconi, B.; Cabras, T.; Pisano, E.; Sanna, M. T.; Olianas, A.; Fanos, V.; Faa, G.; Nemolato, S.; Iavarone, F.; Castagnola, M.; Messana, I. (2013)
Modifications of the acidic soluble salivary proteome in human children from birth to the age of 48 months investigated by a top-down HPLC-ESI-MS platform
in Journal of proteomics (Print)
(literal)

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Manconi, B.; Cabras, T.; Pisano, E.; Sanna, M. T.; Olianas, A.; Fanos, V.; Faa, G.; Nemolato, S.; Iavarone, F.; Castagnola, M.; Messana, I. (literal)

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University of Cagliari; University of Cagliari; Catholic University of the Sacred Heart; Consiglio Nazionale delle Ricerche (CNR) (literal)

Titolo

Modifications of the acidic soluble salivary proteome in human children from birth to the age of 48 months investigated by a top-down HPLC-ESI-MS platform (literal)

Abstract

Biological significance (literal)
During the first year of life the infant oral environment undergoes dramatic changes. To investigate how the salivary proteome of human children evolves during infant development we have analyzed whole saliva of 88 children aged between 0 and 48 months by a top-down platform based on RP-HPLC-ESI-MS. Children were divided according to their age into five groups (A, 0-6 months, N = 17; B, 7-12 months, N = 14; C, 13-24 months, N = 32; D, 25-36 months, N = 16; E, 37-48 months, N = 9). The proteins and peptides analyzed were histatins (histatin-1, histatin-3 1/24), acidic proline-rich proteins, statherin, P-B peptide, and salivary cystatins. Protein and peptide quantification based on the area of the RP-HPLC-E SI-MS extracted ion current peak evidenced that: (i) concentrations of the major salivary proteins/peptides showed a minimum in the 0-6-month-old group and increased with age; (ii) the level of histatin-1 reached a maximum in the 7-12-month-old group, a minimum in the 13-24-month-aged babies and it increased again in the 25-36-month-old group; (iii) S-type cystatins were almost undetectable in the 0-6-month-old group; (iv) P-B peptide concentration greatly increased with age; (v) histatin-3 1/24 and statherin concentrations did not show any age-related variation. (literal)

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