CCD imaging of the electrical activity in the leech nervous system (Articolo in rivista)

Type
Label
  • CCD imaging of the electrical activity in the leech nervous system (Articolo in rivista) (literal)
Anno
  • 1996-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1007/BF00576708 (literal)
Alternative label
  • Canepari Marco, Campani Marco, Spadavecchia Luciano, Torre Vincent (1996)
    CCD imaging of the electrical activity in the leech nervous system
    in European biophysics journal
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Canepari Marco, Campani Marco, Spadavecchia Luciano, Torre Vincent (literal)
Pagina inizio
  • 359 (literal)
Pagina fine
  • 370 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 24 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali
  • 12 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
  • 6 (literal)
Note
  • ISI Web of Science (WOS) (literal)
  • S (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • UNIV GENOA,DIPARTIMENTO FIS,I-16146 GENOA,ITALY; INFM,I-16146 GENOA,ITALY; CNR,IST CIBERNET & BIOFIS,I-16146 GENOA,ITALY (literal)
Titolo
  • CCD imaging of the electrical activity in the leech nervous system (literal)
Abstract
  • A single ganglion of the nervous system of the leech Hirudo medicinalis was isolated. One or both roots emerging from each side of the ganglion were sucked into suction pipettes used either for extracellular stimulation or for recording the gross electrical activity. The ganglion was stained with the fluorescence voltage sensitive dye Di-4-Anepps. The fluorescence was measured with a nitrogen cooled CCD camera. Our recording system allowed us to measure in real time slow optical signals corresponding to changes in light intensity of at least 5 parts per thousand. These signals were caused by the direct polarization of neuronal structures, the afterhyperpolarization or the afterdischarge induced by a prolonged stimulation. When images were acquired at fixed times, several of them could be averaged and optical signals of at least 2 parts per thousand could be reliably measured. These optical signals originated from well identified neurons, such as T, P and N sensory neurons. By taking images at different times and at different focal planes, electrical events could be followed at a temporal resolution of 50 Hz. The three dimensional dynamics of electrical events, initiated by a specific stimulation, was imaged and the spread of excitation among leech neurons was followed. When two roots were selectively stimulated, their neuronal interactions could be imaged and the linear and non-linear terms of the interaction could be characterized. (literal)
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