Autofluorescence properties of rat liver under hyper metabolic conditions. (Articolo in rivista)

Type
Label
  • Autofluorescence properties of rat liver under hyper metabolic conditions. (Articolo in rivista) (literal)
Anno
  • 2007-01-01T00:00:00+01:00 (literal)
Alternative label
  • Croce AC, De Simone U, Vairetti M, Ferrigno A, Bottiroli G. (2007)
    Autofluorescence properties of rat liver under hyper metabolic conditions.
    in Photochemical & photobiological sciences (Print)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Croce AC, De Simone U, Vairetti M, Ferrigno A, Bottiroli G. (literal)
Pagina inizio
  • 1202 (literal)
Pagina fine
  • 1209 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 6 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note
  • DOI: 10.1039/b707309g Work supported by CNR: Target Project “Biotechnology” and by MIUR “COFIN 2006”, Prot. 2006064975_002. (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • (VM; FA)Department of Internal Medicine and Therapeutics, University of Pavia; (CAC;DSU; BG) Istochemistry and Cytometry section, Institute of Molecular Genetics, Consiglio Nazionale delle Ricerche, 27100 Pavia,Italy (literal)
Titolo
  • Autofluorescence properties of rat liver under hyper metabolic conditions. (literal)
Abstract
  • Autofluorescence response to oxygen supply modulation has been investigated in livers of rats under the hypermetabolic state associated to a pathological condition – hyperthyroidism – that is known to enhance hepatocyte metabolic activities involving both NAD, i.e. oxidative pathways engaged in ATP synthesis, and NADP, i.e. reductive bio-synthesis and antioxidant functions. Experiments have been performed on rats in normal condition or submitted to long-term thyroxine (T4) administration. Histological inspection did not evidence any appreciable morphological alteration in liver parenchima; biochemical analysis indicated an increase in both NADP+ and NADPH contents. Autofluorescence properties have been monitored in vivo, via fiber optic probe, on exposed livers both during induction of global ischemia and after restoration of blood circulation. Alteration of oxygen supply modulated liver autofluorescence properties, mainly as to NAD(P)H contribution, in dependence of changes in pyridine coenzymes redox state. With respect to euthyroid, hyperthyroid rat livers exhibited higher autofluorescence signals in all phases of the experiment, and a faster signal decay time upon reoxygenation. The results have been interpreted on the basis of a larger content of NADPH – the coenzyme not directly oxidized in respiratory processes and likely providing an almost constant autofluorescence background contribution - and of uncoupling effects facilitating the respiratory NADH oxidation, associated to hyperthyroid condition. The results obtained in the liver hypermetabolic model provide interesting perspectives for a further improvement of the diagnostic implications of autofluorescence. (literal)
Prodotto di
Autore CNR
Insieme di parole chiave

Incoming links:


Prodotto
Autore CNR di
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi
Insieme di parole chiave di
data.CNR.it