Apoptosis in tumour cells photosensitized with Rose Bengal acetate is induced by multiple organelle photodamage. (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Apoptosis in tumour cells photosensitized with Rose Bengal acetate is induced by multiple organelle photodamage. (Articolo in rivista) (literal)

Anno

• 2007-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1007/s00418-007-0333-3 (literal)

Alternative label

• Soldani C., Croce A.C., Bottone M.G., Fraschini A., Biggiogera M., Bottiroli G., Pellicciari C. (2007)
  Apoptosis in tumour cells photosensitized with Rose Bengal acetate is induced by multiple organelle photodamage.
  in Histochemistry and cell biology
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Soldani C., Croce A.C., Bottone M.G., Fraschini A., Biggiogera M., Bottiroli G., Pellicciari C. (literal)

Pagina inizio

• 485 (literal)

Pagina fine

• 495 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 128 (literal)

Rivista

• Histochemistry and cell biology (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note

• Work partially supported by MIUR (PRIN 2005058254) (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• (SC; FA; BM; BG; PC) Department of Animal Biology, University of Pavia, Piazza Botta 10, 27100, Pavia, and (BMG; BM; CAC; BG) Istochemistry and Cytometry section, Institute of Molecular Genetics, Consiglio Nazionale delle Ricerche, 27100 Pavia,Italy (literal)

Titolo

• Apoptosis in tumour cells photosensitized with Rose Bengal acetate is induced by multiple organelle photodamage. (literal)

Abstract

• Rose Bengal (RB) is a very efficient photosensitizer which undergoes inactivation of its photophysical and photochemical properties upon addition of a quencher group-i.e. acetate-to the xanthene rings. The resulting RB acetate (RB-Ac) derivative behaves as a fluorogenic substrate: it easily enters the cells where the native photoactive molecule is restored by esterase activities. It is known that the viability of RB-Ac-loaded cells is strongly reduced by light irradiation, attesting to the formation of intracellular RB. The aim of this study was to identify the organelles photodamaged by the intracellularly formed RB. RB-Ac preloaded rat C6 glioma cells and human HeLa cells were irradiated at 530 nm. Fluorescence confocal imaging and colocalization with specific dyes showed that the restored RB molecules redistribute dynamically through the cytoplasm, with the achievement of a dynamic equilibrium at 30 min after the administration, in the cell systems used; this accounted for a generalized damage to several organelles and cell structures (i.e. the endoplasmic reticulum, the Golgi apparatus, the mitochondria, and the cytoskeleton). The multiple organelle damage, furthermore, led preferentially to apoptosis as demonstrated by light and electron microscopy and by dual-fluorescence staining with FITC-labelled annexin V and propidium iodide. (literal)

Prodotto di

• Institute of molecular genetics (IGM) (Istituto)
• Sviluppi metodologici in citometria e applicazioni alla sperimentazione biomedica; in vitro imaging (ME.P06.010.001) (Modulo)

Autore CNR

• GIOVANNI BOTTIROLI (Persona)
• Maria Grazia Bottone (Persona)
• ANNA CLETA CROCE (Persona)
• Marco Biggiogera (Unità di personale esterno)

Insieme di parole chiave

• Keywords of "Apoptosis in tumour cells photosensitized with Rose Bengal acetate is induced by multiple organelle photodamage." (Insieme di parole chiave)

Incoming links:

Prodotto

• Institute of molecular genetics (IGM) (Istituto)
• Sviluppi metodologici in citometria e applicazioni alla sperimentazione biomedica; in vitro imaging (ME.P06.010.001) (Modulo)

Autore CNR di

• GIOVANNI BOTTIROLI (Persona)
• ANNA CLETA CROCE (Persona)
• Marco Biggiogera (Unità di personale esterno)
• Maria Grazia Bottone (Persona)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Histochemistry and cell biology (Rivista)

Insieme di parole chiave di

• Keywords of "Apoptosis in tumour cells photosensitized with Rose Bengal acetate is induced by multiple organelle photodamage." (Insieme di parole chiave)