Subnuclear localization and dynamics of the pre-mRNA 3' end processing factor CF Im68. (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Subnuclear localization and dynamics of the pre-mRNA 3' end processing factor CF Im68. (Articolo in rivista) (literal)

Anno

• 2007-01-01T00:00:00+01:00 (literal)

Alternative label

• Cardinale S, Cisterna B, Bonetti P, Aringhieri C, Biggiogera M, Barabino SML. (2007)
  Subnuclear localization and dynamics of the pre-mRNA 3' end processing factor CF Im68.
  in Molecular biology of the cell
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Cardinale S, Cisterna B, Bonetti P, Aringhieri C, Biggiogera M, Barabino SML. (literal)

Pagina inizio

• 1282 (literal)

Pagina fine

• 1292 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 18 (literal)

Rivista

• Molecular biology of the cell (Rivista)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• (CS; BP; AC; BSML) Department of Biotechnology and Biosciences, University of Milano-Bicocca, I-20126 Milan, Italy; (CB; BM) Department of Animal Biology, Laboratory of Cell Biology and Neurobiology, and ( BM) Istochemistry and Cytometry section, Institute of Molecular Genetics, Consiglio Nazionale delle Ricerche, 27100 Pavia,Italy. (literal)

Titolo

• Subnuclear localization and dynamics of the pre-mRNA 3' end processing factor CF Im68. (literal)

Abstract

• Mammalian cleavage factor I (CF Im) is an essential factor that is required for the first step in pre-mRNA 3' end processing. Here, we characterize CF Im68 subnuclear distribution and mobility. Fluorescence microscopy reveals that in addition to paraspeckles CF Im68 accumulates in structures that partially overlap with nuclear speckles. Analysis of synchronized cells shows that CF Im68 distribution in speckles and paraspeckles varies during the cell cycle. At an ultrastructural level, CF Im68 is associated with perichromatin fibrils, the sites of active transcription, and concentrates in interchromatin granules-associated zones. We show that CFIm68 colocalizes with bromouridine, RNA polymerase II, and the splicing factor SC35. On inhibition of transcription, endogenous CF Im68 no longer associates with perichromatin fibrils, but it can still be detected in interchromatin granules-associated zones. These observations support the idea that not only splicing but also 3' end processing occurs cotranscriptionally. Finally, fluorescence recovery after photobleaching analysis reveals that the CF Im68 fraction associated with paraspeckles moves at a rate similar to the more dispersed molecules in the nucleoplasm, demonstrating the dynamic nature of this compartment. These findings suggest that paraspeckles are a functional compartment involved in RNA metabolism in the cell nucleus. (literal)

Prodotto di

• Institute of molecular genetics (IGM) (Istituto)
• Sviluppi metodologici in citometria e applicazioni alla sperimentazione biomedica; in vitro imaging (ME.P06.010.001) (Modulo)

Autore CNR

• Marco Biggiogera (Unità di personale esterno)

Insieme di parole chiave

• Keywords of "Subnuclear localization and dynamics of the pre-mRNA 3' end processing factor CF Im68." (Insieme di parole chiave)

Incoming links:

Prodotto

• Institute of molecular genetics (IGM) (Istituto)
• Sviluppi metodologici in citometria e applicazioni alla sperimentazione biomedica; in vitro imaging (ME.P06.010.001) (Modulo)

Autore CNR di

• Marco Biggiogera (Unità di personale esterno)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Molecular biology of the cell (Rivista)

Insieme di parole chiave di

• Keywords of "Subnuclear localization and dynamics of the pre-mRNA 3' end processing factor CF Im68." (Insieme di parole chiave)