Antioxidant treatments counteract the non-culturability of bacterial endophytes isolated from legume nodules. (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Antioxidant treatments counteract the non-culturability of bacterial endophytes isolated from legume nodules. (Articolo in rivista) (literal)

Anno

• 2013-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1007/s00203-013-0886-6 (literal)

Alternative label

• Muresu R, Tondello A, Polone E, Sulas L, Baldan B, Squartini A. (2013)
  Antioxidant treatments counteract the non-culturability of bacterial endophytes isolated from legume nodules.
  in Archives of microbiology; Springer, Berlin (Germania)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Muresu R, Tondello A, Polone E, Sulas L, Baldan B, Squartini A. (literal)

Pagina inizio

• 385 (literal)

Pagina fine

• 391 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#altreInformazioni

• Arch Microbiol. 2013 Jun;195(6):385-91. doi: 10.1007/s00203-013-0886-6. Epub 2013 Apr 10. (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url

• http://link.springer.com/article/10.1007%2Fs00203-013-0886-6 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 195 (literal)

Rivista

• Archives of microbiology (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

• 7 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 6 (literal)

Note

• PubMe (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• C.N.R., Istituto per il Sistema Produzione Animale in Ambiente Mediterraneo ISPAAM, Traversa La Crucca 3, Località Baldinca, Li Punti, Sassari, Italy. (literal)

Titolo

• Antioxidant treatments counteract the non-culturability of bacterial endophytes isolated from legume nodules. (literal)

Abstract

• In many wild legumes, attempts to cultivate nodule bacteria fail. We hypothesized that the limited culturability could be related to injury from oxidative stress caused by disruption of plant tissues during isolation. To test that, we isolated bacteria from nodules of Hedysarum spinosissimum and Tetragonolobus purpureus using buffers supplemented with scavenging systems to prevent damage from reactive oxygen species (ROS). Treatments included the following: antioxidants (glutathione, ascorbate, EDTA) or enzymes (catalase, peroxidase, superoxide dismutase), tested either as modified squashing buffers or added in plates. Some combinations yielded dramatic increases of culturability. Different endophytes were found, including additional Rhizobiaceae that were not the primary symbiont and were unable to nodulate. Their H2O2 tolerance in broth culture showed differences consistent with the unequal culturability observed. In wild legumes species, ROS generation during extraction appears to be a major factor limiting microbiota isolation, and protocols presented here significantly improve the recovery of culturable bacterial endophytes from plants. (literal)

Editore

• Springer (Editore)

Prodotto di

• Studi bioagronomici per il multiuso di pascoli e risorse foraggere mediterranee (AG.P04.004.001) (Modulo)
• Institute for animal production system in mediterranean environment (ISPAAM) (Istituto)

Autore CNR

• LEONARDO SULAS (Persona)
• ROSELLA MURESU (Unità di personale interno)

Insieme di parole chiave

• Keywords of "Antioxidant treatments counteract the non-culturability of bacterial endophytes isolated from legume nodules." (Insieme di parole chiave)

Incoming links:

Autore CNR di

• ROSELLA MURESU (Unità di personale interno)
• LEONARDO SULAS (Persona)

Prodotto

• Institute for animal production system in mediterranean environment (ISPAAM) (Istituto)
• Studi bioagronomici per il multiuso di pascoli e risorse foraggere mediterranee (AG.P04.004.001) (Modulo)

Editore di

• Springer (Editore)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Archives of microbiology (Rivista)

Insieme di parole chiave di

• Keywords of "Antioxidant treatments counteract the non-culturability of bacterial endophytes isolated from legume nodules." (Insieme di parole chiave)