Raman micro-spectroscopy: A powerful tool for the monitoring of dynamic supramolecular changes in living cells (Articolo in rivista)

Type
Label
  • Raman micro-spectroscopy: A powerful tool for the monitoring of dynamic supramolecular changes in living cells (Articolo in rivista) (literal)
Anno
  • 2013-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1016/j.bpc.2013.06.013 (literal)
Alternative label
  • S. Caponi, Liguori, L., Giugliarelli, A., Mattarelli, M., Morresi, A., Sassi, P., Urbanelli, L., Musio, C (2013)
    Raman micro-spectroscopy: A powerful tool for the monitoring of dynamic supramolecular changes in living cells
    in Biophysical chemistry (Print)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • S. Caponi, Liguori, L., Giugliarelli, A., Mattarelli, M., Morresi, A., Sassi, P., Urbanelli, L., Musio, C (literal)
Pagina inizio
  • 58 (literal)
Pagina fine
  • 63 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url
  • http://www.sciencedirect.com/science/article/pii/S0301462213001026?via=ihub (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 182 (literal)
Rivista
Note
  • ISI Web of Science (WOS) (literal)
  • Scopus (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • a Istituto di Biofisica, Consiglio Nazionale delle Ricerche, c/o Fondazione Bruno Kessler, Via alla Cascata 56/C, 38123 Trento, Italy b Dipartimento di Fisica, Università di Trento, Via Sommarive 14, 38050 Povo, Trento, Italy c Equipe SyNaBi, Laboratoire TIMC UMR CNRS 5525, Université J Fourier, Domaine de la merci, 38700 La Tronche, France d Dipartimento di Chimica, Università di Perugia, Via A. Pascoli, I-06100 Perugia, Italy e NiPS Laboratory, Dipartimento di Fisica, Università di Perugia, Via A. Pascoli, I-06100 Perugia, Italy f Dipartimento di Medicina Sperimentale e Scienze Biochimiche, Università di Perugia, Via del Giochetto, I-06123 Perugia, Italy (literal)
Titolo
  • Raman micro-spectroscopy: A powerful tool for the monitoring of dynamic supramolecular changes in living cells (literal)
Abstract
  • Cellular imaging techniques have become powerful tools in cell biology. With respect to others, the techniques based on vibrational spectroscopy present a clear advantage: the molecular composition and the modification of subcellular compartments can be obtained in label-free conditions. In fact, from the evolution of positions, intensities and line widths of Raman and infrared bands in the cell spectra, characteristic information on cellular activities can be achieved, and particularly, cellular death can be investigated. In this work we present the time evolution of the Raman spectra of single live Jurkat cells (T-lymphocyte) by looking at the high frequency part of their Raman spectra, that is the CH stretching region, around 3000 cm- 1. In particular, investigation into the composition or rearrangement of CH bounds, markers of cellular membrane fatty acids, can represent an important method to study and to recognize cell death. The experimental procedure we used, together with the analysis of these high frequency vibrational bands, may represent a new, improved and advantageous approach to this kind of study. (literal)
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