Top-down HPLC-ESI-MS characterization of rat gliadoralin A, a new member of the family of rat submandibular gland glutamine-rich proteins and potential substrate of transglutaminase (Articolo in rivista)

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  • Top-down HPLC-ESI-MS characterization of rat gliadoralin A, a new member of the family of rat submandibular gland glutamine-rich proteins and potential substrate of transglutaminase (Articolo in rivista) (literal)
Anno
  • 2013-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1002/jssc.201300312 (literal)
Alternative label
  • Tiziana Cabras; Federica Iavarone; Davide Pirolli; Maria Cristina De Rosa; Alberto Vitali; Gavino Faa; Massimo Cordaro; Irene Messana; Jorgen Ekstrom; Massimo Castagnola2,3 (2013)
    Top-down HPLC-ESI-MS characterization of rat gliadoralin A, a new member of the family of rat submandibular gland glutamine-rich proteins and potential substrate of transglutaminase
    in Journal of separation science (Print)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Tiziana Cabras; Federica Iavarone; Davide Pirolli; Maria Cristina De Rosa; Alberto Vitali; Gavino Faa; Massimo Cordaro; Irene Messana; Jorgen Ekstrom; Massimo Castagnola2,3 (literal)
Pagina inizio
  • 2848 (literal)
Pagina fine
  • 2861 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 36 (literal)
Rivista
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  • 17 (literal)
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  • Univ Cagliari, Dipartimento Sci Vita & Ambiente, Cagliari, Italy Univ Cattolica Sacro Cuore, Ist Biochim & Biochim Clin, Fac Med, I-00168 Rome, Italy CNR, Ist Chim Riconoscimento Mol, Rome, Italy Univ Cagliari, Dipartimento Sci Chirurg, Sez Patol, Cagliari, Italy Univ Cattolica Sacro Cuore, Ist Clin Odontoiatr, Fac Med, I-00168 Rome, Italy Univ Gothenburg, Dept Pharmacol, Inst Neurosci & Physiol, Sahlgrenska Acad, Gothenburg, Sweden Univ Cagliari, Dipartimento Sci Biomed, Cagliari, Italy (literal)
Titolo
  • Top-down HPLC-ESI-MS characterization of rat gliadoralin A, a new member of the family of rat submandibular gland glutamine-rich proteins and potential substrate of transglutaminase (literal)
Abstract
  • During HPLC-ESI-MS/MS analysis of rat submandibular saliva secreted under isoprenaline stimulation, a protein with an experimental [M+H](1+) = 10544.24 m/z was detected (17.5 +/- 0.7 min). The MS/MS fragmentation pattern, manually investigated, allowed establishing an internal sequence in agreement with a DNA-derived sequence of an unknown rat protein coded D3Z9M3 (Swiss-Prot). To match the experimental MS/MS fragmentation pattern and protein mass with theoretical data, the removal from the N terminus of the signal peptide and from the C terminus of three amino acid (a.a.) residues (Arg-Ala-Val) and the cyclization of the N-terminal glutamine in pyroglutamic had to be supposed, resulting in a mature protein of 90 a.a. HPLC-ESI-MS/MS of the trypsin digest ensured 100% sequence coverage. For the high glutamine content (34/90 = 37.8%) we propose to name this protein rat gliadoralin A 1-90. Low amounts of five different isoforms were sporadically detected, which did not significantly change their relative amounts after stimulation. Gliadoralin A is substrate for transglutaminase-2, having Lys 60 and different Gln residues as major determinants for enzyme recognition. In silico investigation of superior structures evidenced that a small part of the protein adopts an -helical fold, whereas large segments are unfolded, suggesting an unordered conformation. (literal)
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