Human replication protein A can suppress the intrinsic in vitro mutator phenotype of human DNA polymerase lambda. (Articolo in rivista)

Type
Label
  • Human replication protein A can suppress the intrinsic in vitro mutator phenotype of human DNA polymerase lambda. (Articolo in rivista) (literal)
Anno
  • 2006-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1093/nar/gkl032 (literal)
Alternative label
  • Maga G, Shevelev I, Villani G, Spadari S, Hubscher U. (2006)
    Human replication protein A can suppress the intrinsic in vitro mutator phenotype of human DNA polymerase lambda.
    in Nucleic acids research
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Maga G, Shevelev I, Villani G, Spadari S, Hubscher U. (literal)
Pagina inizio
  • 1405 (literal)
Pagina fine
  • 1415 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 34 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#descrizioneSinteticaDelProdotto
  • Pubblicazione (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Unversity of Zürich-Irchel, Zürich; Ist. di Genetica Molecolare IGM-CNR, Pavia (literal)
Titolo
  • Human replication protein A can suppress the intrinsic in vitro mutator phenotype of human DNA polymerase lambda. (literal)
Abstract
  • DNA polymerase lambda (pol lambda) is a member of the X family DNA polymerases and is endowed with multiple enzymatic activities. In this work we investigated the in vitro miscoding properties of full-length, human pol lambda either in the absence or in the presence of the human auxiliary proteins proliferating cell nuclear antigen (PCNA) and replication protein A (RP-A). Our data suggested that (i) pol lambda had an intrinsic ability to create mismatches and to incorporate ribonucleotides at nearly physiological Mn++ and Mg++ concentrations; (ii) the sequence of the template-primer could influence the misincorporation frequency of pol lambda; (iii) pol lambda preferentially generated G:T and G:G mismatches; (iv) RP-A, but not PCNA, selectively prevented misincorporation of an incorrect nucleotide by pol lambda, without affecting correct incorporation and (v) this inhibitory effect required a precise ratio between the concentrations of pol lambda and RP-A. Possible physiological implications of these findings for the in vivo fidelity of pol lambda are discussed. (literal)
Prodotto di
Autore CNR
Insieme di parole chiave

Incoming links:


Prodotto
Autore CNR di
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi
Insieme di parole chiave di
data.CNR.it