In situ assessment of oxidant and nitrogenic stress in bleomycin pulmonary fibrosis. (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• In situ assessment of oxidant and nitrogenic stress in bleomycin pulmonary fibrosis. (Articolo in rivista) (literal)

Anno

• 2006-01-01T00:00:00+01:00 (literal)

Alternative label

• Inghilleri S, Morbini P, Oggionni T, Barni S, Fenoglio C. (2006)
  In situ assessment of oxidant and nitrogenic stress in bleomycin pulmonary fibrosis.
  in Histochemistry and cell biology
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Inghilleri S, Morbini P, Oggionni T, Barni S, Fenoglio C. (literal)

Pagina inizio

• 661 (literal)

Pagina fine

• 669 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 125 (literal)

Rivista

• Histochemistry and cell biology (Rivista)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• I.F. 2005: 2.239 (literal)

Titolo

• In situ assessment of oxidant and nitrogenic stress in bleomycin pulmonary fibrosis. (literal)

Abstract

• Reactive oxygen species (ROS) and nitric oxide (NO) have a role in the development of pulmonary fibrosis after bleomycin administration. The ROS production induces an antioxidant response, involving superoxide dismutases (SODs), catalase, and glutathione peroxidases. We compared in situ oxidative burden and antioxidant enzyme activity in bleomycin-injured rat lungs and normal controls. ROS expression and catalase, glucose-6-phosphate-dehydrogenase (G6PHD), and NOS/NADPH-diaphorase activity were investigated by using histochemical reactions. Nitric oxide synthase (e-NOS and i-NOS) and SOD (MnSOD, Cu/ZnSOD, ECSOD) expression was investigated immunohistochemically. After treatment ROS production was enhanced in both phagocytes and in type II alveolar epithelial cells. Mn, Cu/Zn, and ECSOD were overexpressed in parenchymal cells, whereas interstitium expressed ECSOD. Catalase and G6PHD activity was moderately increased in parenchymal and inflammatory cells. NOS/NADPH-d activity and i-NOS expression increased in alveolar and bronchiolar epithelia and in inflammatory cells. It can be suggested that the concomitant activation of antioxidant enzymes is not adequate to scavenge the oxidant burden induced by bleomycin lung damage. Inflammatory cells and also epithelial cells are responsible of ROS and NO production. This oxidative and nitrosative stress may be a substantial trigger in TGF-beta1 overexpression by activated type II pneumocytes, leading to fibrotic lesions. (literal)

Prodotto di

• Sviluppi metodologici in citometria e applicazioni alla sperimentazione biomedica; in vitro imaging (ME.P06.010.001) (Modulo)
• Institute of molecular genetics (IGM) (Istituto)

Autore CNR

• Carla Fenoglio (Unità di personale esterno)

Insieme di parole chiave

• Keywords of "In situ assessment of oxidant and nitrogenic stress in bleomycin pulmonary fibrosis." (Insieme di parole chiave)

Incoming links:

Prodotto

• Institute of molecular genetics (IGM) (Istituto)
• Sviluppi metodologici in citometria e applicazioni alla sperimentazione biomedica; in vitro imaging (ME.P06.010.001) (Modulo)

Autore CNR di

• Carla Fenoglio (Unità di personale esterno)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Histochemistry and cell biology (Rivista)

Insieme di parole chiave di

• Keywords of "In situ assessment of oxidant and nitrogenic stress in bleomycin pulmonary fibrosis." (Insieme di parole chiave)