Small ribosomal subunits associate with nuclear myosin and actin in transit to the nuclear pores. (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Small ribosomal subunits associate with nuclear myosin and actin in transit to the nuclear pores. (Articolo in rivista) (literal)

Anno

• 2006-01-01T00:00:00+01:00 (literal)

Alternative label

• Cisterna B, Necchi D, Prosperi E, Biggiogera M. (2006)
  Small ribosomal subunits associate with nuclear myosin and actin in transit to the nuclear pores.
  in The FASEB journal
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Cisterna B, Necchi D, Prosperi E, Biggiogera M. (literal)

Pagina inizio

• 1901 (literal)

Pagina fine

• 1903 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 20 (literal)

Rivista

• ?The ?FASEB journal (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note

• I.F. (2005): 7.064 (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Dipartimento di Biologia Animale, Università di Pavia; Istituto di Genetica Molecolare del CNR, Pavia (literal)

Titolo

• Small ribosomal subunits associate with nuclear myosin and actin in transit to the nuclear pores. (literal)

Abstract

• We have followed at high resolution the ribosomal protein S6 entering the nucleus of HeLa cells, stopping in some (not all) interchromatin granules clusters and reaching, via Cajal bodies, the nucleolus. There, S6 is assembled with other proteins and rRNA into small ribosomal subunit (SSU), released in the nucleoplasm, and exported through the nuclear pores. We show for the first time the spatial association of nuclear myosin I (NMI) and actin with the SSU already at the nucleolar periphery to the nuclear pore. A blockade of NMI or actin induces an upstream accumulation of the S6 protein en route to the nucleolus, and a temperature lower than normal influences RNA export. Our data strongly suggest a functional relationship of SSU with NMI and actin. In our hypothesis, an active, myosin-driven movement of the small ribosomal subunit can be responsible for the export of approximately 10% of SSUs. This hypothesis is supported by ultrastructural, immunofluorescence, and biochemical analyses. The currently accepted model for the subunit release suggests a diffusive, temperature-independent mechanism. However, the advantage of the double mechanism would assure that the movement of a part of the subunits could be modulated, increased, or decreased according to the needs of the cell at a specific moment in the cell cycle. (literal)

Prodotto di

• Sviluppi metodologici in citometria e applicazioni alla sperimentazione biomedica; in vitro imaging (ME.P06.010.001) (Modulo)
• Institute of molecular genetics (IGM) (Istituto)

Autore CNR

• Marco Biggiogera (Unità di personale esterno)
• ENNIO PROSPERI (Persona)

Insieme di parole chiave

• Parole chiave di "Small ribosomal subunits associate with nuclear myosin and actin in transit to the nuclear pores." (Insieme di parole chiave)

Incoming links:

Prodotto

• Institute of molecular genetics (IGM) (Istituto)
• Sviluppi metodologici in citometria e applicazioni alla sperimentazione biomedica; in vitro imaging (ME.P06.010.001) (Modulo)

Autore CNR di

• ENNIO PROSPERI (Persona)
• Marco Biggiogera (Unità di personale esterno)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• ?The ?FASEB journal (Rivista)

Insieme di parole chiave di

• Parole chiave di "Small ribosomal subunits associate with nuclear myosin and actin in transit to the nuclear pores." (Insieme di parole chiave)