Immunohistochemical localization of dentin matrix protein 1 in human dentin (Articolo in rivista)

Type
Label
  • Immunohistochemical localization of dentin matrix protein 1 in human dentin (Articolo in rivista) (literal)
Anno
  • 2008-01-01T00:00:00+01:00 (literal)
Alternative label
  • Orsini G, Ruggeri A, Mazzoni A, Nato F, Falconi M, Putignano A, Di Lenarda R, Nanci A, Breschi L. (2008)
    Immunohistochemical localization of dentin matrix protein 1 in human dentin
    in European journal of histochemistry (Testo stamp.)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Orsini G, Ruggeri A, Mazzoni A, Nato F, Falconi M, Putignano A, Di Lenarda R, Nanci A, Breschi L. (literal)
Pagina inizio
  • 215 (literal)
Pagina fine
  • 220 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 52 (literal)
Rivista
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Department of Restorative Dentistry, Institute of Stomatology, Polytechnique University of Marche, Ancona, Italy; Department of SAU&FAL, University of Bologna, Italy; Unit of Dental Sciences and Biomaterials, Department of Biomedicine, University of Trieste, Italy; Faculty of Dentistry, University of Montréal, Canada; IGM-CNR, Unit of Bologna c/o IOR, Bologna, Italy (literal)
Titolo
  • Immunohistochemical localization of dentin matrix protein 1 in human dentin (literal)
Abstract
  • Dentin matrix protein 1 (DMP1) is a non-collagenous matrix protein with a recognized role in the formation of mineralized tissues such as dentin. The aim of this study was to analyze the distribution of DMP1 in human dentin by means of immunofluorescence and high-resolution immunogold labeling. Fully developed, sound human dentin specimens were submitted to fluorescence labeling and post-embedding immunolabeling techniques with a rabbit polyclonal antihuman DMP1 antibody followed by corresponding fluorochrome- conjugated or gold-conjugated secondary antibodies. Both immunofluorescence and immunogold labeling showed an intense labeling associated with the peritubular dentin. In addition, at the ultrastructural level, there was also a moderate and diffuse immunoreaction over intertubular dentin, and a weak labeling within predentin which increased in density towards the mineralization front. This study suggests that in adult human teeth, like in rodents, DMP1 is prevalently concentrated at the level of peritubular dentin and this feature is preserved also in fully developed-teeth. These data are consistent with what has been observed in rodents and suggest that DMP1 plays a role in maintenance of the dentin tubular space. (literal)
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