Enhanced Adsorption of Ca-ATPase Containing Vesicles on a Negatively Charged Solid-Supported-Membrane for the Investigation of Membrane Transporters (Articolo in rivista)

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  • Enhanced Adsorption of Ca-ATPase Containing Vesicles on a Negatively Charged Solid-Supported-Membrane for the Investigation of Membrane Transporters (Articolo in rivista) (literal)
Anno
  • 2013-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1021/la4034386 (literal)
Alternative label
  • Alessio Sacconi (1); Maria Rosa Moncelli (1); Giancarlo Margheri (2); Francesco Tadini-Buoninsegni (1) (2013)
    Enhanced Adsorption of Ca-ATPase Containing Vesicles on a Negatively Charged Solid-Supported-Membrane for the Investigation of Membrane Transporters
    in Langmuir; ACS, American chemical society, Washington, DC (Stati Uniti d'America)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Alessio Sacconi (1); Maria Rosa Moncelli (1); Giancarlo Margheri (2); Francesco Tadini-Buoninsegni (1) (literal)
Pagina inizio
  • 13883 (literal)
Pagina fine
  • 13889 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url
  • http://pubs.acs.org/doi/abs/10.1021/la4034386 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 29 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali
  • 7 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
  • 45 (literal)
Note
  • Scopu (literal)
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • (1) Department of Chemistry \"Ugo Schiff\", University of Florence, via della Lastruccia 3, 50019 Sesto Fiorentino, Italy (2) Institute for Complex Systems, National Research Council, Via Madonna del Piano 10, 50019 Sesto Fiorentino, Italy (literal)
Titolo
  • Enhanced Adsorption of Ca-ATPase Containing Vesicles on a Negatively Charged Solid-Supported-Membrane for the Investigation of Membrane Transporters (literal)
Abstract
  • A convenient model system for a biological membrane is a solid-supported membrane (SSM), which consists of a gold-supported alkanethiol|phospholipid bilayer. In combination with a concentration jump method, SSMs have been used for the investigation of several membrane transporters. Vesicles incorporating sarcoplasmic reticulum Ca-ATPase (SERCA) were adsorbed on a negatively charged SSM (octadecanethiol|phosphatidylserine bilayer). The current signal generated by the adsorbed vesicles following an ATP concentration jump was compared to that produced by SERCA-containing vesicles adsorbed on a conventional SSM (octadecanethiol|phosphatidylcholine bilayer). A significantly higher current amplitude was recorded on the serine-based SSM. The adsorption of SERCA-incorporating vesicles on the SSM was then characterized by surface plasmon resonance (SPR). The SPR measurements clearly indicate that in the presence of Ca2+ and Mg2+, the amount of adsorbed vesicles on the serine-based SSM is about twice that obtained using the conventional SSM, thereby demonstrating that the higher current amplitude recorded on the negatively charged SSM is correlated with a greater quantity of adsorbed vesicles. The enhanced adsorption of membrane vesicles on the PS-based SSM may be useful to study membrane preparations with a low concentration of transport protein generating small current signals, as in the case of various recombinantly expressed proteins. (literal)
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