Digital holography as a method for 3D imaging and estimating biovolume of motile cells (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Digital holography as a method for 3D imaging and estimating biovolume of motile cells (Articolo in rivista) (literal)

Anno

• 2013-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1039/C3LC50515D (literal)

Alternative label

• F. Merola, L. Miccio, P. Memmolo, G. Di Caprio, A. Galli, R. Puglisi, D. Balduzzi, G. Coppola, P. Netti, P. Ferraro (2013)
  Digital holography as a method for 3D imaging and estimating biovolume of motile cells
  in Lab on a chip (Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• F. Merola, L. Miccio, P. Memmolo, G. Di Caprio, A. Galli, R. Puglisi, D. Balduzzi, G. Coppola, P. Netti, P. Ferraro (literal)

Pagina inizio

• 4512 (literal)

Pagina fine

• 4516 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 13 (literal)

Rivista

• Lab on a chip (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

• 5 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 23 (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• CNR-INO, Via Campi Flegrei 34, Pozzuoli (NA) 80078, Italy IIT-CRIB, L.go Barsanti e Matteucci 53, Napoli 80125, Italy CNR-IMM, Via P. Castellino 111, Napoli 80131, Italy Istituto \"L. Spallanzani\", Rivolta d'Adda, Cremona 26027, Italy (literal)

Titolo

• Digital holography as a method for 3D imaging and estimating biovolume of motile cells (literal)

Abstract

• Sperm morphology is regarded as a significant prognostic factor for fertilization, as abnormal sperm structure is one of the most common factors in male infertility. Furthermore, obtaining accurate morphological information is an important issue with strong implications in zoo-technical industries, for example to perform sorting of species X from species Y. A challenging step forward would be the availability of a fast, high-throughput and label-free system for the measurement of physical parameters and visualization of the 3D shape of such biological specimens. Here we show a quantitative imaging approach to estimate simply and quickly the biovolume of sperm cells, combining the optical tweezers technique with digital holography, in a single and integrated set-up for a biotechnology assay process on the lab-on-a-chip scale. This approach can open the way for fast and high-throughput analysis in label-free microfluidic based \"cytofluorimeters\" and prognostic examination based on sperm morphology, thus allowing advancements in reproductive science. (literal)

Prodotto di

• Institute for microelectronics and microsystems (IMM) (Istituto)
• Istituto di cibernetica \"Edoardo Caianiello\" (ISASI) (Istituto)
• Sviluppo di tecniche di diagnostica ottica, microscopia ed interferometria (MD.P03.022.001) (Modulo)

Autore CNR

• FRANCESCO MEROLA (Persona)
• GIUSEPPE DI CAPRIO (Persona)
• Pasquale Memmolo (Persona)
• GIUSEPPE COPPOLA (Persona)
• LISA MICCIO (Unità di personale interno)
• PIETRO FERRARO (Unità di personale interno)

Incoming links:

Autore CNR di

• PIETRO FERRARO (Unità di personale interno)
• GIUSEPPE DI CAPRIO (Persona)
• Pasquale Memmolo (Persona)
• LISA MICCIO (Unità di personale interno)
• GIUSEPPE COPPOLA (Persona)
• FRANCESCO MEROLA (Persona)

Prodotto

• Institute for microelectronics and microsystems (IMM) (Istituto)
• Istituto di cibernetica \"Edoardo Caianiello\" (ISASI) (Istituto)
• Sviluppo di tecniche di diagnostica ottica, microscopia ed interferometria (MD.P03.022.001) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Lab on a chip (Rivista)