Label-free biochemical characterization of bovine sperm cells using Raman microscopy (Contributo in atti di convegno)

Type

• Contributo in atti di convegno (Classe)
• Prodotto della ricerca (Classe)

Label

• Label-free biochemical characterization of bovine sperm cells using Raman microscopy (Contributo in atti di convegno) (literal)

Anno

• 2013-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1117/12.2021575 (literal)

Alternative label

• Anna Chiara De Luca; Stefano Manago; Maria A. Ferrara; Luigi Sirleto; Roberto Puglisi; Donatella Balduzzi; Andrea Galli; Ivo Rendina; Pietro Ferraro; Giuseppe Coppola (2013)
  Label-free biochemical characterization of bovine sperm cells using Raman microscopy
  in Optical Methods for Inspection, Characterization, and Imaging of Biomaterials, Munich, Germany, May 13, 2013
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Anna Chiara De Luca; Stefano Manago; Maria A. Ferrara; Luigi Sirleto; Roberto Puglisi; Donatella Balduzzi; Andrea Galli; Ivo Rendina; Pietro Ferraro; Giuseppe Coppola (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#titoloVolume

• Optical Methods for Inspection, Characterization, and Imaging of Biomaterials (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#volumeInCollana

• 8792 (literal)

Rivista

• Proceedings of SPIE (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Istituto di Biochimica delle Proteine, CNR (Italy) Istituto per la Microelettronica e Microsistemi, CNR (Italy) Istituto Sperimentale Italiano Lazzaro Spallanzani (Italy) Istituto Nazionale di Ottica, CNR (Italy) (literal)

Titolo

• Label-free biochemical characterization of bovine sperm cells using Raman microscopy (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#isbn

• 9780819496089 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autoriVolume

• Pietro Ferraro; Monika Ritsch-Marte; Simonetta Grilli; David Stifter, (literal)

Abstract

• Raman spectroscopy is a label-free and non-invasive method that measures the inelastic scattered light from a sample giving insight into the vibration eigenmodes of the excited molecules. For these reasons, Raman spectroscopy has been used as a powerful tool to investigate different biological tissues and living cells. In this paper, we present a Raman spectroscopy-based method for sensitive biochemical characterization of bovine sperm cells. Importantly, by analysing separate Raman spectra from the nucleus, acrosomale vesicle and tail of single sperm cells, we are able to identify characteristic Raman features associated with DNA, protein and lipid molecular vibrations for discriminating among different locations inside the cell with sub-micrometric resolution (~0.3 ?m). We demonstrate that our Raman spectroscopy facilitates spectral assignment and increases detection sensitivity, opening the way for novel bio-imaging platforms. © (2013) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only. (literal)

Editore

• SPIE-INT SOC OPTICAL ENGINEERING (Editore)

Prodotto di

• Istituto di cibernetica \"Edoardo Caianiello\" (ISASI) (Istituto)
• Institute of protein biochemistry (IBP) (Istituto)
• Microsistemi optoelettronici in silicio e tecnologie compatibili (MD.P05.014.001) (Modulo)
• Institute for microelectronics and microsystems (IMM) (Istituto)

Autore CNR

• MARIA ANTONIETTA FERRARA (Persona)
• LUIGI SIRLETO (Persona)
• IVO RENDINA (Unità di personale interno)
• PIETRO FERRARO (Unità di personale interno)
• ANNA CHIARA DE LUCA (Persona)
• GIUSEPPE COPPOLA (Persona)

Incoming links:

Autore CNR di

• PIETRO FERRARO (Unità di personale interno)
• MARIA ANTONIETTA FERRARA (Persona)
• LUIGI SIRLETO (Persona)
• IVO RENDINA (Unità di personale interno)
• GIUSEPPE COPPOLA (Persona)
• ANNA CHIARA DE LUCA (Persona)

Prodotto

• Institute for microelectronics and microsystems (IMM) (Istituto)
• Istituto di cibernetica \"Edoardo Caianiello\" (ISASI) (Istituto)
• Institute of protein biochemistry (IBP) (Istituto)
• Microsistemi optoelettronici in silicio e tecnologie compatibili (MD.P05.014.001) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Proceedings of SPIE (Rivista)

Editore di

• SPIE-INT SOC OPTICAL ENGINEERING (Editore)