Neurosphere and neurosphere-forming cells: morphological and ultrastructural characterization. (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Neurosphere and neurosphere-forming cells: morphological and ultrastructural characterization. (Articolo in rivista) (literal)

Anno

• 2003-01-01T00:00:00+01:00 (literal)

Alternative label

• Bez A, Corsini E, Curti D, Biggiogera M, Colombo A, Nicosia RF, Pagano SF, Parati EA. (2003)
  Neurosphere and neurosphere-forming cells: morphological and ultrastructural characterization.
  in Brain research
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Bez A, Corsini E, Curti D, Biggiogera M, Colombo A, Nicosia RF, Pagano SF, Parati EA. (literal)

Pagina inizio

• 18 (literal)

Pagina fine

• 29 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 993 (literal)

Rivista

• Brain research (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note

• Ricercatore afferente al CNR- Ricercatore associato: M. Biggioggera- Impact Factor: 2.414 (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Laboratory of Neurobiology, Department of Neurobiology and Neurorestorative Therapies, National Neurological Institute “C. Besta”, Milan, Italy; Department of Physiological Science and Cellular and Molecular Pharmacology, University of Pavia, Pavia, Italy; Department of Animal Biology, University of Pavia, Pavia, Italy; Institute for Obstetric and Gynaecology \"L. Mangiagalli\", ICP, Milan, Italy e Veterans Affairs Puget Sound Health Care System, Seattle, WA, USA; Department of Pathology, University of Washington, Seattle, WA, USA (literal)

Titolo

• Neurosphere and neurosphere-forming cells: morphological and ultrastructural characterization. (literal)

Abstract

• Despite recent advances in our understanding of neural stem cell (NSC) biology, the free-floating structures generated by these cells in vitro, the \"neurospheres\", have not been fully characterized. To fill this gap, we examined neurospheres and neurosphere-derived NSCs by confocal microscopy, electron microscopy (EM) and cytofluorimetry. Here, we show that neurospheres and neurosphere-forming cells are morphologically and functionally heterogeneous. Confocal microscopy reveals differences in cell size, viability, cytoplasmic content and in the presence and distribution of active mitochondria. By electron microscopy, neurospheres appear as complex structures in which biological events such as mitosis, apoptosis and even phagocytosis are influenced by NSCs localization within the architecture of the neurosphere. NSCs derived from neurospheres are not synchronized and are represented in all phases of the cell cycle. Cytofluorimetric studies demonstrate NSCs' heterogeneity in cell size by forward scatter (FSC) analysis, and in cytoplasmic granularity by side scatter (SSC) profiling. These findings may contribute to our understanding of the morphogenesis of the neurospheres, particularly as this process relates to the high environmental adaptability of the NSCs and the reported existence of different subpopulations of neural stem cells. (literal)

Prodotto di

• Institute of molecular genetics (IGM) (Istituto)

Autore CNR

• Marco Biggiogera (Persona)

Incoming links:

Prodotto

• Institute of molecular genetics (IGM) (Istituto)

Autore CNR di

• Marco Biggiogera (Persona)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Brain research (Rivista)