Top-down HPLC-ESI-MS detection of S-Glutathionylated and S-Cysteinylated Derivatives of Cystatin B and Its 1-53 and 54-98 Fragments in Whole Saliva of Human Preterm Newborns (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Top-down HPLC-ESI-MS detection of S-Glutathionylated and S-Cysteinylated Derivatives of Cystatin B and Its 1-53 and 54-98 Fragments in Whole Saliva of Human Preterm Newborns (Articolo in rivista) (literal)

Anno

• 2013-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1021/pr300960f (literal)

Alternative label

• Iavarone, Federica; Cabras, Tiziana; Pisano, Elisabetta; Sanna, Maria Teresa; Nemolato, Sonia; Vento, Giovanni; Tirone, Chiara; Romagnoli, Costantino; Cordaro, Massimo; Fanos, Vassilios; Faa, Gavino; Messana, Irene; Castagnola, Massimo (2013)
  Top-down HPLC-ESI-MS detection of S-Glutathionylated and S-Cysteinylated Derivatives of Cystatin B and Its 1-53 and 54-98 Fragments in Whole Saliva of Human Preterm Newborns
  in Journal of proteome research (Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Iavarone, Federica; Cabras, Tiziana; Pisano, Elisabetta; Sanna, Maria Teresa; Nemolato, Sonia; Vento, Giovanni; Tirone, Chiara; Romagnoli, Costantino; Cordaro, Massimo; Fanos, Vassilios; Faa, Gavino; Messana, Irene; Castagnola, Massimo (literal)

Pagina inizio

• 917 (literal)

Pagina fine

• 926 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 12 (literal)

Rivista

• Journal of proteome research (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

• 10 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 2 (literal)

Note

• ISI Web of Science (WoS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Catholic University of the Sacred Heart; Consiglio Nazionale delle Ricerche (CNR); University of Cagliari; University of Cagliari; Catholic University of the Sacred Heart; Catholic University of the Sacred Heart (literal)

Titolo

• Top-down HPLC-ESI-MS detection of S-Glutathionylated and S-Cysteinylated Derivatives of Cystatin B and Its 1-53 and 54-98 Fragments in Whole Saliva of Human Preterm Newborns (literal)

Abstract

• Analysis by a HPLC-ESI-MS top-down proteornic platform of specimens of human preterm newborn whole saliva evidenced high relative amounts of cystatin B and its S-glutathionylated, S-cysteinylated, and S-S 2-mer (on Cys(3),) derivatives, decreasing as a function of postconceptional age (PCA). The percentage of S-unmodified cystatin B was higher than the S-modified isoforms in the early PCA period, differently from adults where cystatin B was detectable only as S-modified derivatives. The percentage of S-modified derivatives increased as a function of PCA, reaching at the normal term of delivery values similar to those determined in at-term newborns, babies, and adults. Moreover, in the early PCA period, high relative amounts of the 1-53 and 54-98 cystatin B fragments were detected, decreasing as a function of PCA and disappearing at the normal term of delivery. In agreement with intact cystatin B, fragment 1-53 was detectable as S-unmodified and S-modified derivatives, and their percentages changed accordingly with the percentages of intact proteins, suggesting that the fragmentation process could be subsequent to and independent from the S-modification of the protein. This study highlights specific enzymatic activity in the oral cavity of preterm newborns not present in at-term newborns and adults, which can be a clue to specialized pathways occurring during fetal oral development. (literal)

Prodotto di

• Istituto di chimica del riconoscimento molecolare (ICRM) (Istituto)
• Proteomica salivare (PM.P06.008.002) (Modulo)

Autore CNR

• MASSIMO CASTAGNOLA (Persona)

Insieme di parole chiave

• Parole chiave di "Top-down HPLC-ESI-MS detection of S-Glutathionylated and S-Cysteinylated Derivatives of Cystatin B and Its 1-53 and 54-98 Fragments in Whole Saliva of Human Preterm Newborns" (Insieme di parole chiave)

Incoming links:

Prodotto

• Istituto di chimica del riconoscimento molecolare (ICRM) (Istituto)
• Proteomica salivare (PM.P06.008.002) (Modulo)

Autore CNR di

• MASSIMO CASTAGNOLA (Persona)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Journal of proteome research (Rivista)

Insieme di parole chiave di

• Parole chiave di "Top-down HPLC-ESI-MS detection of S-Glutathionylated and S-Cysteinylated Derivatives of Cystatin B and Its 1-53 and 54-98 Fragments in Whole Saliva of Human Preterm Newborns" (Insieme di parole chiave)