8-oxoguanine incorporation into DNA repeats in vitro and mismatch recognition by MutSalpha. (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• 8-oxoguanine incorporation into DNA repeats in vitro and mismatch recognition by MutSalpha. (Articolo in rivista) (literal)

Anno

• 2005-01-01T00:00:00+01:00 (literal)

Alternative label

• Macpherson P., Barone F., Maga G., Mazzei F., Karran P., Bignami M. (2005)
  8-oxoguanine incorporation into DNA repeats in vitro and mismatch recognition by MutSalpha.
  in Nucleic acids research
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Macpherson P., Barone F., Maga G., Mazzei F., Karran P., Bignami M. (literal)

Pagina inizio

• 5094 (literal)

Pagina fine

• 5105 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 33 (literal)

Rivista

• Nucleic acids research (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note

• Impact Factor = 7.260 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#descrizioneSinteticaDelProdotto

• pubblicazione scientifica (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Macpherson, Karran: Cancer Research UK, London; Barone, Mazzei, Bignami: Ist. Superiore di Sanità, Roma. Maga: IGM-CNR (literal)

Titolo

• 8-oxoguanine incorporation into DNA repeats in vitro and mismatch recognition by MutSalpha. (literal)

Abstract

• DNA 8-oxoguanine (8-oxoG) causes transversions and is also implicated in frameshifts. We previously identified the dNTP pool as a likely source of mutagenic DNA 8-oxoG and demonstrated that DNA mismatch repair prevented oxidation-related frameshifts in mononucleotide repeats. Here, we show that both Klenow fragment and DNA polymerase alpha can utilize 8-oxodGTP and incorporate the oxidized purine into model frameshift targets. Both polymerases incorporated 8-oxodGMP opposite C and A in repetitive DNA sequences and efficiently extended a terminal 8-oxoG. The human MutSalpha mismatch repair factor recognized DNA 8-oxoG efficiently in some contexts that resembled frameshift intermediates in the same C or A repeats. DNA 8-oxoG in other slipped/mispaired structures in the same repeats adopted configurations that prevented recognition by MutSalpha and by the OGG1 DNA glycosylase thereby rendering it invisible to DNA repair. These findings are consistent with a contribution of oxidative DNA damage to frameshifts. They also suggest how mismatch repair might reduce the burden of DNA 8-oxoG and prevent frameshift formation. (literal)

Prodotto di

• Sviluppo e meccanismo d'azione di analoghi nucleotidici e nucleosidici come composti antiproliferativi e antivirali : nuovi composti e nuovi bersagli per la terapia (ME.P04.004.001) (Modulo)
• Institute of molecular genetics (IGM) (Istituto)

Autore CNR

• GIOVANNI MAGA (Unità di personale interno)

Insieme di parole chiave

• Keywords of "8-oxoguanine incorporation into DNA repeats in vitro and mismatch recognition by MutSalpha." (Insieme di parole chiave)

Incoming links:

Prodotto

• Institute of molecular genetics (IGM) (Istituto)
• Sviluppo e meccanismo d'azione di analoghi nucleotidici e nucleosidici come composti antiproliferativi e antivirali : nuovi composti e nuovi bersagli per la terapia (ME.P04.004.001) (Modulo)

Autore CNR di

• GIOVANNI MAGA (Unità di personale interno)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Nucleic acids research (Rivista)

Insieme di parole chiave di

• Keywords of "8-oxoguanine incorporation into DNA repeats in vitro and mismatch recognition by MutSalpha." (Insieme di parole chiave)