http://www.cnr.it/ontology/cnr/individuo/prodotto/ID272120

Tailored hydrogel membranes for efficient protein crystallization (Articolo in rivista)

Type

Label

Tailored hydrogel membranes for efficient protein crystallization (Articolo in rivista) (literal)

Anno

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Alternative label

Di Profio, G., Polino, M., Nicoletta, F. P., Belviso, B. D., Caliandro, R., Fontananova, E., De Filpo, G., Curcio, E., Drioli, E. (2014)
Tailored hydrogel membranes for efficient protein crystallization
in Advanced functional materials (Print)
(literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

Di Profio, G., Polino, M., Nicoletta, F. P., Belviso, B. D., Caliandro, R., Fontananova, E., De Filpo, G., Curcio, E., Drioli, E. (literal)

Pagina inizio

Pagina fine

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Rivista

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Note

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National Research Council of Italy (CNR), Institute on Membrane Technology (ITM), Via P. Bucci c/o Università della Calabria Cubo 17/C, 87036 Rende (CS), Italy. University of Calabria, Department of Pharmacy, Health and Nutritional Sciences, Via P. Bucci Ed. Polifunzionale, 87036 Rende (CS), Italy. University of Calabria, Department of Chemistry and Chemical Technologies, Via P. Bucci Cubo 15/C, 87036 Rende (CS), Italy. National Research Council of Italy (CNR), Institute of Crystallography (IC), Via P. Amendola 122/o, 70125 Bari (BA), Italy. University of Calabria, Department of Environmental and Chemical Engineering, Via P. Bucci Cubo 15/C, 87036 Rende (CS), Italy. (literal)

Titolo

Abstract

Crystallization still represents the bottleneck in the process of protein structure determination at high resolution, despite high-throughput structural genomics programs require optimized crystallization strategies regarding crystal quality, time, success rate, reproducibility and used protein amount. On the other side, the development of suitable materials for controlled heterogeneous nucleation might facilitate biomacromolecular crystallization in a variety of experimental conditions which are not conventionally fruitful. Here we show the possibility to fabricate hydrogel membranes displaying controlled chemical composition and nanostructure and to use them as heterogeneous supports for biomacromolecular crystallization. Diverse gel morphologies were obtained by controlling phase separation kinetics during gel layer formation on membrane support. These composite materials were found to increase the efficiency of the crystallization process so that crystals with enhanced diffraction properties were produced at lower protein concentration than conventional technique, thus affording the possibility to improve current approaches to protein crystallization and to be adapted to specific targets. (literal)

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