http://www.cnr.it/ontology/cnr/individuo/prodotto/ID271678

Surface immobilized hydrogels as versatile reagent reservoirs for microarrays (Articolo in rivista)

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Surface immobilized hydrogels as versatile reagent reservoirs for microarrays (Articolo in rivista) (literal)

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Sola, Laura; Gagni, Paola; Cretich, Marina; Chiari, Marcella (2013)
Surface immobilized hydrogels as versatile reagent reservoirs for microarrays
in Journal of immunological methods (Print)
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Titolo

Surface immobilized hydrogels as versatile reagent reservoirs for microarrays (literal)

Abstract

The accuracy of antibody-based microarrays depends on eliminating or at least minimizing the effect of cross-reactive components. In this work, a method to create a versatile, compartmentalized storage system for antibodies and soluble microarray reagents is described. These containers are made of hydrogel plugs immobilized on a slide facing the corresponding sub-array on a printed surface. (literal)
The hydrogels were polymerized using a 4% w/v N,N-dimethylacrylamide and 3% w/v bisacrylamide solution, thus generating large pore sizes to allow the facile transfer of intact, functional biomolecules. The hydrogel microenvironments can be desiccated and rehydrated with a desired solution, and they can store reagents in a dry form. (literal)

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