Use of epididymal semen cryoconservation in farm animal gene banking: field applications in Brianzola sheep and Nero Siciliano pig breeds. (Abstract/Poster in rivista)

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  • Use of epididymal semen cryoconservation in farm animal gene banking: field applications in Brianzola sheep and Nero Siciliano pig breeds. (Abstract/Poster in rivista) (literal)
Anno
  • 2013-01-01T00:00:00+01:00 (literal)
Alternative label
  • Pizzi F, Turri F, Gliozzi TM , Gandini G (2013)
    Use of epididymal semen cryoconservation in farm animal gene banking: field applications in Brianzola sheep and Nero Siciliano pig breeds.
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Pizzi F, Turri F, Gliozzi TM , Gandini G (literal)
Pagina inizio
  • 83 (literal)
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  • Poster presentato al XX ASPA Congress, Bologna 11-13 June 2013. L'abstract è stato pubblicato sulla rivista Italian Journal of Animal Science 2013; volume 12 s1 83. (literal)
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  • 12 (literal)
Rivista
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  • s1 (literal)
Note
  • Abstract (literal)
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  • FP, FT, TMG - IBBA CNR, Milano; GG DIVET UNIMI. (literal)
Titolo
  • Use of epididymal semen cryoconservation in farm animal gene banking: field applications in Brianzola sheep and Nero Siciliano pig breeds. (literal)
Abstract
  • In local breeds, farmed in marginal area, few males are available for semen collection on farm. Semen collection training would be more complicated considering that the animals are not accustomed to being handled by humans. Moreover cryopreservation procedures are complex for the lack of facilities and expertise near the farming area. In this context epididymal sperm extraction from testicles of slaughtered or castrated animals is the only option to collect gametes. The aim of this work was to implement epididymal extraction technique, associated to the possibility of managing rather long periods between animal death, sperm recovery and freezing, for the setting up of two Italian local breeds' genetic reserve: the Brianzola sheep (BS) and the Nero Siciliano pig (NSP) breeds. In case of BS, testicles from 9 rams were collected at local slaughterhouse and semen samples extracted and frozen in our laboratories. For NSP, testicles from 10 boars were collected on farm in Sicily, after surgical castration. For 5 boars epididymal sperm recovery and freezing were done on-site; for the remaining boars testicles were transported by air at 5°C and processed within 24 hours in our laboratories in Lodi. Epididymal sperm was collected by using the retrograde flushing technique. After dilution samples were equilibrated at 5°C for 2 h, subsequently loaded into 0.5 mL straws and frozen on nitrogen vapors. Semen volume, total number of sperm and sperm motility were assessed. A total of 207 and 115 semen doses were stored for BS and NSP breed, respectively. Overall the quality of the sperm recovered was acceptable, considering semen volume (BS =.45± 0.4 ml; NSP=9.69±5.8 mL), total number of sperm collected (BS=4.7±4.05x109 cells/mL; NSP= 26.0±32.1x109 cells/mL) and total motility (BS=30.9±8.0%; NSP=63.0±7.5%). The high variability among samples detected depends mainly on donor age. Access to new technologies is important for gene banks to develop their collections. These data suggest the possibility to extract viable epididymal sperm from testicles transported and processed within 24 hours. (literal)
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