http://www.cnr.it/ontology/cnr/individuo/prodotto/ID271456

Cloning and expression of synthetic genes encoding angiotensin-I converting enzyme (ACE)-inhibitory bioactive peptides in Bifidobacterium pseudocatenulatum (Articolo in rivista)

Type

Articolo in rivista (Classe)
Prodotto della ricerca (Classe)

Label

Cloning and expression of synthetic genes encoding angiotensin-I converting enzyme (ACE)-inhibitory bioactive peptides in Bifidobacterium pseudocatenulatum (Articolo in rivista) (literal)

Anno

2013-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

10.1111/1574-6968.12068 (literal)

Alternative label

Luca Losurdo 1,2,4, Laura Quintieri 3, Leonardo Caputo 3, Raffaele Gallerani 1, Baltasar Mayo2, Francesca De Leo 4,* (2013)
Cloning and expression of synthetic genes encoding angiotensin-I converting enzyme (ACE)-inhibitory bioactive peptides in Bifidobacterium pseudocatenulatum
in FEMS Microbiology Letters; Federation of European Microbiological Societies (FEMS), Reading (Regno Unito)
(literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

Luca Losurdo 1,2,4, Laura Quintieri 3, Leonardo Caputo 3, Raffaele Gallerani 1, Baltasar Mayo2, Francesca De Leo 4,* (literal)

Pagina inizio

24 (literal)

Pagina fine

32 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#altreInformazioni

This study was partially supported by the Italian Ministry of Education, University and Research (MIUR) (Project FIRB-MERIT 2008 No. RBNE08HWLZ_012 \"Basi molecolari nelle sindromi degenerative correlate con l'invecchiamento\") and by the Spanish Ministry of Economy and Competitiveness to B.M. (ref. AGL2011-24300-ALI). Luca Losurdo was the recipient of a fellowship from the Italian Consortium for Biotechnologies. Part of the activities was also carried out within the Research project 'Development of innovative food products through biotechnological, plant design and technological solutions' (PROINNO_BIT) funded by Italian Ministry of Education, University and Research (MIUR). The authors would like to thank Dr Federico Baruzzi for his critical reading of the manuscript. (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

340 (literal)

Rivista

FEMS Microbiology Letters (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

9 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

1 (literal)

Note

ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

1 Dipartimento di Bioscienze, Biotecnologie e Scienze Farmacologiche, Università degli Studi di Bari 'Aldo Moro', Bari, Italy 2 Departamento de Microbiología y Bioquímica, Instituto de Productos Lácteos (IPLA-CSIC), Villaviciosa, Asturias, Spain 3 Istituto di Scienze delle Produzioni Alimentari (ISPA), Consiglio Nazionale delle Ricerche (CNR), Bari, Italy 4 Istituto di Biomembrane e Bioenergetica (IBBE), Consiglio Nazionale delle Ricerche (CNR), Bari, Italy (literal)

Titolo

Cloning and expression of synthetic genes encoding angiotensin-I converting enzyme (ACE)-inhibitory bioactive peptides in Bifidobacterium pseudocatenulatum (literal)

Abstract

A wide range of biopeptides potentially able to lower blood pressure through inhibition of the angiotensin-I converting enzyme (ACE) is produced in fermented foods by proteolytic starter cultures. This work applies a procedure based on recombinant DNA technologies for the synthesis and expression of three ACE-inhibitory peptides using a probiotic cell factory. ACE-inhibitory genes and their pro-active precursors were designed, synthesized by PCR, and cloned in Escherichia coli; after which, they were cloned into the pAM1 E. coli-bifidobacteria shuttle vector. After E. coli transformation, constructs carrying the six recombinant clones were electrotransferred into the Bifidobacterium pseudocatenulatum M115 probiotic strain. Interestingly, five of the six constructs proved to be stable. Their expression was confirmed by reverse transcription PCR. Furthermore, transformed strains displayed ACE-inhibitory activity linearly correlated to increasing amounts of cell-free cellular lysates. In particular, 50 ?g of lysates from constructs pAM1-Pro-BP3 and pAM1-BP2 showed a 50% higher ACE-inhibitory activity than that of the controls. As a comparison, addition of 50 ng of Pro-BP1 and Pro-BP3 synthetic peptides to 50 ?g of cell-free extracts of B. pseudocatenulatum M115 wild-type strain showed an average of 67% of ACE inhibition; this allowed estimating the amount of the peptides produced by the transformants. Engineering of bifidobacteria for the production of biopeptides is envisioned as a promising cell factory model system. (literal)

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