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Stability and fusion of lipid layers on polyelectrolyte multilayer supports studied by colloidal force spectroscopy (Articolo in rivista)

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Stability and fusion of lipid layers on polyelectrolyte multilayer supports studied by colloidal force spectroscopy (Articolo in rivista) (literal)

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Kohler, G; Moya, SE; Leporatti, S; Bitterlich, C; Donath, E (2007)
Stability and fusion of lipid layers on polyelectrolyte multilayer supports studied by colloidal force spectroscopy
in European biophysics journal
(literal)

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Univ Leipzig, Fac Med, Inst Med Phys & Biophys, D-04107 Leipzig, Germany; CiCbiomagune, San Sebastian 20009, Gipuzkoa, Spain; CNR, INFM, Natl Nanotechnol Lab, I-73100 Lecce, Italy (literal)

Titolo

Stability and fusion of lipid layers on polyelectrolyte multilayer supports studied by colloidal force spectroscopy (literal)

Abstract

The interaction between lipid layers supported by polyelectrolyte multilayer cushions has been studied by means of colloidal force spectroscopy. In a typical experiment, a colloidal probe engineered with a layer-by-layer film and a lipid bilayer on top is approached to a planar surface coated in a symmetrical way. Kinks of a few nanometres in width appear when lipid layers are pressed together-reflecting either fusion processes between lipid layers or membranes, or the penetration of polymer blobs into or through the lipid layers. Retracting curves show a stepwise shape, which results from lipid tether formation or from polymer stretching, the latter suggesting that polyelectrolyte multilayers make contact as a result of penetration or lipid fusion. (literal)

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