Preservation of mitochondrial functional integrity in mitochondria isolated from small cryopreserved mouse brain areas (Articolo in rivista)

Type
Label
  • Preservation of mitochondrial functional integrity in mitochondria isolated from small cryopreserved mouse brain areas (Articolo in rivista) (literal)
Anno
  • 2014-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1016/j.ab.2013.08.030 (literal)
Alternative label
  • Valenti Daniela, de Bari Lidia, De Filippis Bianca, Ricceri Laura, Vacca Rosa Anna (2014)
    Preservation of mitochondrial functional integrity in mitochondria isolated from small cryopreserved mouse brain areas
    in Analytical biochemistry (Print); ELSEVIER, NEW YORK (Stati Uniti d'America)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Valenti Daniela, de Bari Lidia, De Filippis Bianca, Ricceri Laura, Vacca Rosa Anna (literal)
Pagina inizio
  • 25 (literal)
Pagina fine
  • 31 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 444 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali
  • 7 (literal)
Note
  • ISI Web of Science (WOS) (literal)
  • Scopu (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Institute of Biomembranes and Bioenergetics, National Council of Research, Bari, Italy; Section of Behavioural Neuroscience, Department of Cell Biology and Neurosciences, Istituto Superiore di Sanità Roma, Italy; Section of Neurotoxicology and Neuroendocrinology, Department of Cell Biology and Neurosciences, Istituto Superiore di Sanità Roma, Italy. (literal)
Titolo
  • Preservation of mitochondrial functional integrity in mitochondria isolated from small cryopreserved mouse brain areas (literal)
Abstract
  • Studies of mitochondrial bioenergetics in brain pathophysiology are often precluded by the need to isolate mitochondria immediately after tissue dissection from a large number of brain biopsies for comparative studies. Here we present a procedure of cryopreservation of small brain areas from which mitochondrial enriched fractions (crude mitochondria) with high oxidative phosphorylation efficiency can be isolated. Small mouse brain areas were frozen and stored in a solution containing glycerol as cryoprotectant. Crude mitochondria were isolated by differential centrifugation from both cryopreserved and freshly explanted brain samples and compared with respect to their ability to generate membrane potential and to produce ATP. Intactness of outer and inner mitochondrial membranes was verified by polarographic ascorbate and cytochrome c tests and spectrophotometric assay of citrate synthase activity. Preservation of structural integrity and oxidative phosphorylation efficiency has been successfully obtained in crude mitochondria isolated from different areas of cryopreserved mouse brain samples. Long-term cryopreservation of small brain areas from which intact and phosphorylating mitochondria can be isolated for the study of mitochondrial bioenergetics, will significantly expand the study of mitochondrial defects in neurological pathologies allowing large comparative studies and favoring inter-laboratory and interdisciplinary analyses. (literal)
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