Curcumin protects against NMDA-induced toxicity: a possible role for NR2A subunit. (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Curcumin protects against NMDA-induced toxicity: a possible role for NR2A subunit. (Articolo in rivista) (literal)

Anno

• 2011-01-01T00:00:00+01:00 (literal)

Alternative label

• Matteucci A, Cammarota R, Paradisi S, Varano M, Balduzzi M, Leo L, Bellenchi GC, De Nuccio C, Carnovale-Scalzo G, Scorcia G, Frank C, Mallozzi C, Di Stasi AM, Visentin S, Malchiodi-Albedi F. (2011)
  Curcumin protects against NMDA-induced toxicity: a possible role for NR2A subunit.
  in Investigative ophthalmology & visual science
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Matteucci A, Cammarota R, Paradisi S, Varano M, Balduzzi M, Leo L, Bellenchi GC, De Nuccio C, Carnovale-Scalzo G, Scorcia G, Frank C, Mallozzi C, Di Stasi AM, Visentin S, Malchiodi-Albedi F. (literal)

Pagina inizio

• 1070 (literal)

Pagina fine

• 1077 (literal)

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• 52(2 (literal)

Rivista

• Investigative ophthalmology & visual science (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note

• PMID: 20861489 (literal)

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• Pubblicazione (literal)

Note

• ISI Web of Science (WOS) (literal)

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• CNR-IGB \"ABT\" (literal)

Titolo

• Curcumin protects against NMDA-induced toxicity: a possible role for NR2A subunit. (literal)

Abstract

• Curcumin, a phenolic compound extracted from the rhizome of Curcuma longa, was found to attenuate NMDA-induced excitotoxicity in primary retinal cultures. This study was conducted to further characterize curcumin neuroprotective ability and analyze its effects on NMDA receptor (NMDAr). METHODS: NMDAr modifications were analyzed in primary retinal cell cultures using immunocytochemistry, whole-cell patch-clamp recording and western blot analysis. Cell death was evaluated with the TUNEL assay in primary retinal and hippocampal cultures. Optical fluorometric recordings with Fura 2-AM were used to monitor [Ca(2+)](i). RESULTS: Curcumin dose- and time-dependently protected both retinal and hippocampal neurons against NMDA-induced cell death, confirming its anti-excitotoxic property. In primary retinal cultures, in line with the observed reduction of NMDA-induced [Ca(2+)](i) rise, whole-cell patch-clamp experiments showed that a higher percentage of retinal neurons responded to NMDA with low amplitude current after curcumin treatment. In parallel, curcumin induced an increase in NMDAr subunit type 2A (NR2A) level, with kinetics closely correlated to time-course of neuroprotection and decrease in [Ca(2+)](i). The relation between neuroprotection and NR2A level increase was also in line with the observation that curcumin neuroprotection required protein synthesis. Electrophysiology confirmed an increased activity of NR2A-containing NMDAr at the plasma membrane level. (literal)

Prodotto di

• Institute of genetics and biophysics \"Adriano Buzzati Traverso\" (IGB) (Istituto)
• Sviluppo e funzionamento dei sistemi complessi - Uso di modelli biologici (SV.P16.005.001) (Modulo)

Autore CNR

• GIAN CARLO BELLENCHI (Unità di personale interno)

Incoming links:

Prodotto

• Institute of genetics and biophysics \"Adriano Buzzati Traverso\" (IGB) (Istituto)
• Sviluppo e funzionamento dei sistemi complessi - Uso di modelli biologici (SV.P16.005.001) (Modulo)

Autore CNR di

• GIAN CARLO BELLENCHI (Unità di personale interno)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Investigative ophthalmology & visual science (Rivista)