Nuclear Aggregates of Polyamines in a radiation-induced DNA damage model (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Nuclear Aggregates of Polyamines in a radiation-induced DNA damage model (Articolo in rivista) (literal)

Anno

• 2014-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1016/j.biocel.2013.11.007 (literal)

Alternative label

• Giuseppe Iacomino, Gianluca Picariello, Ilaria Stillitano, Luciano D'Agostino (2014)
  Nuclear Aggregates of Polyamines in a radiation-induced DNA damage model
  in International journal of biochemistry & cell biology
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Giuseppe Iacomino, Gianluca Picariello, Ilaria Stillitano, Luciano D'Agostino (literal)

Pagina inizio

• 11 (literal)

Pagina fine

• 19 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url

• http://www.sciencedirect.com/science/article/pii/S1357272513003452 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 47 (literal)

Rivista

• International journal of biochemistry & cell biology (Rivista)

Note

• PubMe (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• ISA-CNR (literal)

Titolo

• Nuclear Aggregates of Polyamines in a radiation-induced DNA damage model (literal)

Abstract

• Polyamines (PA) are believed to protect DNA minimizing the effect of radiation damage either by inducing DNA compaction and aggregation or acting as scavengers of free radicals. Using an in vitro pDNA double strand breakage assay based on gel electrophoretic mobility, we compared the protective capability of PA against ?-radiation with that of compounds generated by the supramolecular self-assembly of nuclear polyamines and phosphates, named Nuclear Aggregates of Polyamines (NAPs). Both unassembled PA and in vitro produced NAPs (ivNAPs) were ineffective in conferring pDNA protection at the sub-mM concentration. Single PA showed an appreciable protective effect only at high (mM) concentrations. However, concentrations of spermine (4+) within a critical range (0.48 - 1 mM) induced pDNA precipitation, an event that was not observed with NAPs-pDNA interaction. We conclude that the interaction of individual PA is ineffective to assure DNA protection, simultaneously preserving the flexibility and charge density of the double strand. Furthermore, data obtained by testing polyamine and ivNAPS with the current radiation-induced DNA damage model support the concept that PA-phosphate aggregates are the only forms through which PA interact with DNA (literal)

Prodotto di

• Tecnologie, Tracciabilità e Sicurezza degli Alimenti (AG.P05.004.001) (Modulo)
• Alimenti e salute dell'uomo (AG.P05.005.001) (Modulo)
• Institute of food sciences (ISA) (Istituto)

Autore CNR

• GIANLUCA PICARIELLO (Persona)
• GIUSEPPE IACOMINO (Persona)
• Luciano D'Agostino (Unità di personale esterno)

Insieme di parole chiave

• Keywords of "Nuclear Aggregates of Polyamines in a radiation-induced DNA damage model" (Insieme di parole chiave)

Incoming links:

Prodotto

• Institute of food sciences (ISA) (Istituto)
• Tecnologie, Tracciabilità e Sicurezza degli Alimenti (AG.P05.004.001) (Modulo)
• Alimenti e salute dell'uomo (AG.P05.005.001) (Modulo)

Autore CNR di

• GIANLUCA PICARIELLO (Persona)
• GIUSEPPE IACOMINO (Persona)
• Luciano D'Agostino (Unità di personale esterno)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• International journal of biochemistry & cell biology (Rivista)

Insieme di parole chiave di

• Keywords of "Nuclear Aggregates of Polyamines in a radiation-induced DNA damage model" (Insieme di parole chiave)