Proteomic approach to protein expression profiles in patients with Common Variable Immunodeficiency (Abstract/Poster in rivista)

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Label
  • Proteomic approach to protein expression profiles in patients with Common Variable Immunodeficiency (Abstract/Poster in rivista) (literal)
Anno
  • 2008-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1111/j.1398-9995.2008.01760.x (literal)
Alternative label
  • Spadaro, G; D'Orio, C; Stranges, S; D'Angelo, F; D'Ambrosio, C; Scaloni, A; Genovese, A; Zambrano, N (2008)
    Proteomic approach to protein expression profiles in patients with Common Variable Immunodeficiency
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Spadaro, G; D'Orio, C; Stranges, S; D'Angelo, F; D'Ambrosio, C; Scaloni, A; Genovese, A; Zambrano, N (literal)
Pagina inizio
  • 476 (literal)
Pagina fine
  • 477 (literal)
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  • http://onlinelibrary.wiley.com/doi/10.1111/j.1398-9995.2008.01760.x/abstract (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 63 (literal)
Rivista
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  • 2 (literal)
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  • s88 (literal)
Note
  • Poster (literal)
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  • University of Naples ''Federico II'', Clinical Immunology and Allergy, Naples, Italy, University of Naples ''Federico II'', CEINGE, Naples, Italy, Consiglio Nazionale delle Ricerche, Proteomics and Mass Spectrometry Laboratory, Naples, Italy (literal)
Titolo
  • Proteomic approach to protein expression profiles in patients with Common Variable Immunodeficiency (literal)
Abstract
  • Common Variable Immunodeficiency (CVID) is the most frequent primary immunodeficiency in adult. CVID is characterized by a marked decrease in serum immunoglobulins and high susceptibility to recurrent bacterial infections of the respiratory and gastrointestinal tracts. The clinical course of CVID can be complicated by autoimmune disorders, neoplasia, lympho proliferative syndromes, or granulomas. Proteomics was defined as the study of complex biosystems (such as serum and tissues) in order to characterize content and changes in their proteome, induced by physiological or pathologic conditions.We used a proteomic approach to study protein expression profiles of serum samples from three patients (2 males, 1 female), (age 12 to 59 years) with CVID, evaluated at the Division of Clinical Immunology and Allergy of the University of Naples ''Federico II''. First, we compared their serum proteomes to control proteomes. Then, we evaluated changes in protein expression after one year of substitutive therapy with intravenous immunoglobulins. We used 2-D differential gel electrophoresis (2D- DIGE), that allows to separate proteins according to their charge and molecular weights. Taking advantage of fluorescent labelling of the samples, dedicated software platforms allow to identify quali-quantitative alterations in protein levels. Compared to controls, patients before treatment show reduced levels of some proteins, already known in literature because of their relationships to infections and cancer. Besides, patients before treatment show higher serum levels of other proteins, that are correlated to inflammatory conditions. Finally, comparing proteome of these patients with CVID before and after one year of substitutive therapy, we observed that therapy actually modifies, and in many instances reverts the observed trends. Our data show that the serum proteome patterns of patients with CVID is different from control proteomes. These differences could characterize specific clinical phenotypes, representing evidence for evolution of immunodeficiency towards infective or neoplastic diseases. Further more, therapy with immunoglobulins changes protein profile expression in these patients, suggesting a possible corrective effect by substitutive therapy in patients with CVID. (literal)
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