Electrospun Polyacrylonitrile Nanofibrous Membranes Tailored for Acetylcholinesterase Immobilization (Articolo in rivista)

Type
Label
  • Electrospun Polyacrylonitrile Nanofibrous Membranes Tailored for Acetylcholinesterase Immobilization (Articolo in rivista) (literal)
Anno
  • 2010-01-01T00:00:00+01:00 (literal)
Alternative label
  • Stoilova O, Manolova N, GABROVSKA K, MARINOV I, GODJEVARGOVA T, MITA DG AND RASHKOV i. (2010)
    Electrospun Polyacrylonitrile Nanofibrous Membranes Tailored for Acetylcholinesterase Immobilization
    in Journal of bioactive and compatible polymers (Print)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Stoilova O, Manolova N, GABROVSKA K, MARINOV I, GODJEVARGOVA T, MITA DG AND RASHKOV i. (literal)
Pagina inizio
  • 40 (literal)
Pagina fine
  • 57 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 25 (literal)
Rivista
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Laboratory of Bioactive Polymers, Institute of Polymers, Bulgarian Academy of Sciences, Acad. G. Bonchev str., 103A, BG-1113 Sofia, Bulgaria University ‘‘Prof. Dr. Asen Zlatarov’’, Department of Biotechnology Prof Y. Yakimov str., 1, BG-8010 Burgas, Bulgaria Laboratory of Biophysics, Institute of Genetics and Biophysics – CNR Pietro Castellino str., 111, 80131 Naples, Italy National Institute of Biosystems and Biostructures (INBB) Via le Medaglie d’Oro, 305, 00136 Rome, Italy (literal)
Titolo
  • Electrospun Polyacrylonitrile Nanofibrous Membranes Tailored for Acetylcholinesterase Immobilization (literal)
Abstract
  • Nanofibrous polyacrylonitrile membranes (PANNFM) were obtained by electrospinning and then prepared for immobilizing acetylcholinesterase (AChE). Initially, the chemical modification of PANNFM with ethylenediamine produced reactive groups to overcome their inertness and hydrophobicity. The natural polymer, chitosan, was then tethered on the nanofibrous membranes to improve their biocompatibility. Scanning electron microscopy (SEM) and crosssection SEM were used to determine morphological and porosity changes of the membranes. The immobilized AChE had greater relative activity as well as thermal and storage stability compared to the free enzyme. The bound AChE showed excellent reusability. Chitosan-modified PANNFM was shown to be a suitable strategy for facile immobilization of AChE to produce a promising system that effectively supports biocatalysts. (literal)
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