http://www.cnr.it/ontology/cnr/individuo/prodotto/ID26901

Small Interfering RNA Induced TLR3 Activation Inhibits Blood and Lymphatic Vessel Growth (Articolo in rivista)

Type

Label

Small Interfering RNA Induced TLR3 Activation Inhibits Blood and Lymphatic Vessel Growth (Articolo in rivista) (literal)

Anno

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

Alternative label

Choa W.; Albuquerque R.J.C.; Kleinman M.E.; Tarallo V.; Greco A.; Nozaki M.; Rich M.G.; Baffi J.Z.; Ambati B.;De Falco M.; Alexander J.S.; Brunetti A.; De Falco S.; and Ambati J. (2009)
Small Interfering RNA Induced TLR3 Activation Inhibits Blood and Lymphatic Vessel Growth
in Proceedings of the National Academy of Sciences of the United States of America; National Academy of Sciences, Washington (Stati Uniti d'America)
(literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

Choa W.; Albuquerque R.J.C.; Kleinman M.E.; Tarallo V.; Greco A.; Nozaki M.; Rich M.G.; Baffi J.Z.; Ambati B.;De Falco M.; Alexander J.S.; Brunetti A.; De Falco S.; and Ambati J. (literal)

Pagina inizio

Pagina fine

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

Rivista

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

Note

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

Departments of aOphthalmology and Visual Sciences and bPhysiology, University of Kentucky, Lexington, KY 40536; Institute of Genetics and Biophysics Adriano Buzzati-Traverso, Consiglio Nazionale delle Ricerche, Naples 80131, Italy; Department of Biomorphological and Functional Sciences, University Federico II, Istituto di Biostrutture e Bioimmagini-Consiglio Nazionale delle Ricerche and Centro di Ingegneria Genetica, Naples 80131, Italy; Department of Ophthalmology and Visual Sciences, Moran Eye Center, University of Utah School of Medicine, Salt Lake City, UT 84132; Department of Ophthalmology, Veterans Affairs Salt Lake City Health Care System, Salt Lake City, UT 84148; Department of Anesthesiological, Surgical and Emergency Sciences, V Unit of Surgery and Advanced Surgical Procedures, Second University of Naples, Naples 80100, Italy; Department of Molecular and Cellular Physiology,Louisiana State University Health Sciences Center, Shreveport, LA 71130-3932 (literal)

Titolo

Small Interfering RNA Induced TLR3 Activation Inhibits Blood and Lymphatic Vessel Growth (literal)

Abstract

Neovascularization in response to tissue injury consists of the dual invasion of blood (hemangiogenesis) and lymphatic (lymphangiogenesis) vessels. We reported recently that 21-nt or longer small interfering RNAs (siRNAs) can suppress hemangiogenesis in mouse models of choroidal neovascularization and dermal wound healing independently of RNA interference by directly activating Toll-like receptor 3 (TLR3), a double-stranded RNA immune receptor, on the cell surface of blood endothelial cells. Here, we show that a 21-nt nontargeted siRNA suppresses both hemangiogenesis and lymphangiogenesis in mouse models of neovascularization induced by corneal sutures or hindlimb ischemia as efficiently as a 21-nt siRNA targeting vascular endothelial growth factor-A. In contrast, a 7-nt nontargeted siRNA, which is too short to activate TLR3, does not block hemangiogenesis or lymphangiogenesis in these models. Exposure to 21-nt siRNA, which we demonstrate is not internalized unless cell-permeating moieties are used, triggers phosphorylation of cell surface TLR3 on lymphatic endothelial cells and induces apoptosis. These findings introduce TLR3 activation as a method of jointly suppressing blood and lymphatic neovascularization and simultaneously raise new concerns about the undesirable effects of siRNAs on both circulatory systems. (literal)

Editore